| Nonantibody-based recognition: alternative molecules for detection of pathogens. | |
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MedLine Citation:
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PMID: 17078765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoassays have been well established for many years as the cornerstone of detection technologies. These assays are sensitive, selective and, in general, highly resistant to interference from complex sample matrices when compared with nucleic acid-based tests. However, both antibody- and nucleic acid-based detection systems require a priori knowledge of the target and development of specific reagents; multiplexed assays can become increasingly problematic when attempting to detect a plethora of different targets, the identities of which are unknown. In an effort to circumvent many of the limitations inherent in these conventional assays, other recognition reagents are being explored as alternatives, or indeed as adjuncts, to antibodies for pathogen and toxin detection. This article will review a number of different recognition systems ranging in complexity from small molecules, such as nucleic-acid aptamers, carbohydrates and peptides, to systems as highly complicated as whole cells and organisms. All of these alternative systems have tremendous potential to achieve superior sensitivity, selectivity, and stability, but are also subject to their own limitations, which are also discussed. In short, while in its infancy, this field holds great promise for the development of rapid, fieldable assays that are highly complementary to existing antibody- and nucleic acid-based technologies. |
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Authors:
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Miriam M Ngundi; Nadezhda V Kulagina; George P Anderson; Chris R Taitt |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Expert review of proteomics Volume: 3 ISSN: 1744-8387 ISO Abbreviation: - Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-11-02 Completed Date: 2006-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101223548 Medline TA: Expert Rev Proteomics Country: England |
Other Details:
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Languages: eng Pagination: 511-24 Citation Subset: IM |
Affiliation:
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US Food and Drug Administration, N29 RM418 HFM-434 8800 Rockville Pike, Bethesda, MD 20892, USA. miriam.ngundi@fda.hhs.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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isolation & purification* Diagnostic Techniques and Procedures* Fungi / isolation & purification* Polymers / diagnostic use Proteomics / methods SELEX Aptamer Technique Toxins, Biological / analysis Viruses / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 0/Toxins, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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