| Non-viral VEGF(165) gene therapy--magnetofection of acoustically active magnetic lipospheres ('magnetobubbles') increases tissue survival in an oversized skin flap model. | |
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MedLine Citation:
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PMID: 19040418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study, we investigated the potential of magnetofection of magnetic lipospheres containing VEGF(165)-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound. We prepared perfluoropropane-filled magnetic lipospheres ('magnetobubbles') from Tween60-coated magnetic nanoparticles, Metafectene, soybean-oil and cDNA and studied the effect in an oversized random-pattern-flap model in the rats (n= 46). VEGF-cDNA-magnetobubbles were administered under a magnetic field with simultaneously applied ultrasound, under magnetic field alone and with applied ultrasound alone. Therapy was conducted 7 days pre-operative. Flap survival and necrosis were measured 7 days post-operatively. Flap perfusion, VEGF-protein concentration in target and surrounding tissue, formation and appearance of new vessels were analysed additionally. Magnetofection with VEGF-cDNA-magnetobubbles presented an increased flap survival of 50% and increased flap perfusion (P < 0.05). Without ultrasound and without magnetic field, the effect is weakened. VEGF concentration in target tissue was elevated (P < 0.05), while underlying muscle was not affected. Our results demonstrate the successful VEGF gene therapy by means of magnetobubble magnetofection. Here, the method of magnetofection of magnetic lipospheres is equally efficient as adenoviral transduction, but has a presumable superior safety profile. |
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Authors:
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Thomas Holzbach; Dialekti Vlaskou; Iva Neshkova; Moritz A Konerding; Klaus Wörtler; Olga Mykhaylyk; Bernd Gänsbacher; H-G Machens; Christian Plank; Riccardo E Giunta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-14 |
Journal Detail:
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Title: Journal of cellular and molecular medicine Volume: 14 ISSN: 1582-4934 ISO Abbreviation: J. Cell. Mol. Med. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101083777 Medline TA: J Cell Mol Med Country: England |
Other Details:
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Languages: eng Pagination: 587-99 Citation Subset: IM |
Affiliation:
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Department of Plastic Surgery and Hand Surgery, Munich, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Enzyme-Linked Immunosorbent Assay Gene Therapy / methods* Graft Survival / physiology* Lipids / chemistry Magnetics Male Microspheres Microvessels / physiology Models, Animal Muscles / metabolism Rats Rats, Sprague-Dawley Skin / metabolism, surgery Skin Transplantation Surgical Flaps / blood supply Transfection / methods* Ultrasonics Vascular Endothelial Growth Factors / genetics, metabolism, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Vascular Endothelial Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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