Document Detail


Non-viable human embryos as a source of viable cells for embryonic stem cell derivation.
MedLine Citation:
PMID:  19192355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human embryonic stem cells (hESC) hold great promise for use in regenerative medicine. However, the extraordinary potential of hESC as therapeutic tools is tempered by ethical, moral and political issues surrounding their derivation from human embryos. It has previously been proposed that ethical criteria applied to essential organ donation could be employed for derivation of hESC from irreversibly arrested, and thus organismically dead, human embryos produced during routine IVF procedures. Here, it is shown that arrested embryos do not resume normal development during extended culture, yet most of them contain a substantial number of living cells on embryonic day 6 (72% have <1 viable cell, 47% have <5 viable cells), suggesting that this class of non-viable embryos could be a rich source of viable cells for derivation of hESC lines.
Authors:
Svetlana Gavrilov; Robert W Prosser; Imran Khalid; Joanne MacDonald; Mark V Sauer; Donald W Landry; Virginia E Papaioannou
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-04     Completed Date:  2009-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  301-8     Citation Subset:  IM    
Affiliation:
Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Cell Separation
Cell Survival / physiology
Cells, Cultured
Cleavage Stage, Ovum / cytology,  physiology
Embryo Culture Techniques
Embryo Disposition*
Embryo, Mammalian / cytology*,  physiology*
Embryonic Development / physiology
Embryonic Stem Cells / cytology*
Humans
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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