Document Detail


Non-uniform membrane probe distribution in resonance energy transfer: application to protein-lipid selectivity.
MedLine Citation:
PMID:  16532364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biological membranes are, at the molecular level, quasi-two dimensional systems. Membrane components are often distributed non-uniformly in the bilayer plane, as a consequence of lipid phase separation/domain formation or local enrichment/depletion of particular lipid species arising form favorable/unfavorable lipid-membrane protein interactions. Due to its explicit dependence on donor-acceptor distance or local acceptor concentration, resonance energy transfer (RET) has large potential in the characterization of membrane heterogeneity. RET formalisms for the basic geometric arrangements relevant for membranes have now been known for several decades. However, these formalisms usually assume uniform distributions, and more general models are required for the study of membrane lateral heterogeneity. We present a model that addresses the possibility of non-uniform acceptor (e.g., lipid probe) distribution around each donor (e.g., protein) in a membrane. It considers three regions with distinct local acceptor concentration, namely, an exclusion zone, the membrane bulk, and, lying in between, a region of enhanced probability of finding acceptors (annular region). Numerical solutions are presented, and convenient empirical fitting functions are given for RET efficiency as a function of bulk acceptor surface concentration, for several values of the model parameters. The usefulness of the formalism is illustrated in the analysis of experimental data.
Authors:
Ricardo C Capeta; José A Poveda; Luís M S Loura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-11
Journal Detail:
Title:  Journal of fluorescence     Volume:  16     ISSN:  1053-0509     ISO Abbreviation:  J Fluoresc     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-05-12     Completed Date:  2007-02-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201341     Medline TA:  J Fluoresc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  161-72     Citation Subset:  IM    
Affiliation:
Centro de Química e Departamento de Química, Universidade de Evora, Evora, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / chemistry
Energy Transfer
Fluorescence Resonance Energy Transfer*
Fluorescent Dyes / analysis*
Membrane Lipids / chemistry*
Membrane Proteins / chemistry*
Models, Chemical*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Membrane Lipids; 0/Membrane Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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