Document Detail


Nonsteady state oxygen transport in engineered tissue: implications for design.
MedLine Citation:
PMID:  23350630     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Engineered tissue constructs are limited in size, and thus clinical relevance, when diffusion is the primary mode of oxygen transport. Understanding the extent of oxygen diffusion and cellular consumption is necessary for the design of engineered tissues, particularly those intended for implantation into hypoxic wound sites. This study presents a combined experimental and computation model to predict design constraints for cellularized fibrin tissues subjected to a step change in the oxygen concentration to simulate transplantation. Nonsteady state analysis of oxygen diffusion and consumption was used to estimate the diffusion coefficient of oxygen (mean±SD, 1.7×10(-9)±8.4×10(-11) m(2)/s) in fibrin hydrogels as well as the Michaelis-Menten parameters, Vmax (1.3×10(-17)±9.2×10(-19) mol·cell(-1)·s(-1)), and Km (8.0×10(-3)±3.5×0(-3) mol/m(3)), of normal human lung fibroblasts. Nondimensionalization of the governing diffusion-reaction equation enabled the creation of a single dimensionless parameter, the Thiele modulus (φ), which encompasses the combined effects of oxygen diffusion, consumption, and tissue dimensions. Tissue thickness is the design parameter with the most pronounced influence on the distribution of oxygen within the system. Additionally, tissues designed such that φ<1 achieve a near spatially uniform and adequate oxygen concentration following the step change. Understanding and optimizing the Thiele modulus will improve the design of engineered tissue implants.
Authors:
Seema M Ehsan; Steven C George
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-03-13
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-29     Completed Date:  2013-11-15     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1433-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Count
Cell Survival / drug effects
Computer Simulation
Diffusion
Elastic Modulus / drug effects
Fibrin / pharmacology
Fibroblasts / cytology,  drug effects
Gels
Humans
Models, Biological
Oxygen / metabolism*
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
RC1 ES018361/ES/NIEHS NIH HHS; UH2 TR000481/TR/NCATS NIH HHS; UH2 TR000481/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/Gels; 9001-31-4/Fibrin; S88TT14065/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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