Document Detail


Non-specific translocation of peptide-displaying bacteriophage particles across the gastrointestinal barrier.
MedLine Citation:
PMID:  18602466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phage display technology could provide a rapid means for the discovery of novel peptides and proteins from genetically engineered variants which may act as specific vehicles for drug delivery particularly through the intestinal barrier. In this work, we utilized in vivo phage display in order to study the sequences which may be responsible for transmucosal transport. We hypothesized that the introduction of a library of peptide displaying phages into the intestine may lead to the identification of sequences that could induce transport. A biopanning protocol was performed by applying a 7-mer random amino acid phage library to mice by gavage and then assessing their absorption via phage recovery from the spleen and blood. Following the isolation of 77 different phages, the sequences of the displayed peptides were identified. Statistical treatment of the obtained sequences did not support the notion that the GI translocation depends on the presence of any particular peptide sequence fused on the pIII coat proteins of the M13 phages. There are, however, some residue types underrepresented which could be due to specific GI selection mechanisms and/or their effects on the amplification rate for phages bearing those residues.
Authors:
Maryam Hamzeh-Mivehroud; Ali Mahmoudpour; Hasan Rezazadeh; Siavoush Dastmalchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-17
Journal Detail:
Title:  European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V     Volume:  70     ISSN:  0939-6411     ISO Abbreviation:  Eur J Pharm Biopharm     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9109778     Medline TA:  Eur J Pharm Biopharm     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  577-81     Citation Subset:  IM    
Affiliation:
School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage M13 / physiology*
Biological Transport
Drug Delivery Systems*
Gastrointestinal Tract / metabolism*
Peptide Library*
Chemical
Reg. No./Substance:
0/Peptide Library

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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