Document Detail


Nonreductive iron uptake mechanism in the marine alveolate Chromera velia.
MedLine Citation:
PMID:  20724644     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromera velia is a newly cultured photosynthetic marine alveolate. This microalga has a high iron requirement for respiration and photosynthesis, although its natural environment contains less than 1 nm of this metal. We found that this organism uses a novel mechanism of iron uptake, differing from the classic reductive and siderophore-mediated iron uptake systems characterized in the model yeast Saccharomyces cerevisiae and present in most yeasts and terrestrial plants. C. velia has no trans-plasma membrane electron transfer system, and thus cannot reduce extracellular ferric chelates. It is also unable to use hydroxamate siderophores as iron sources. Iron uptake from ferric citrate by C. velia is not inhibited by a ferrous chelator, but the rate of uptake is strongly decreased by increasing the ferric ligand (citrate) concentration. The cell wall contains a large number of iron binding sites, allowing the cells to concentrate iron in the vicinity of the transport sites. We describe a model of iron uptake in which aqueous ferric ions are first concentrated in the cell wall before being taken up by the cells without prior reduction. We discuss our results in relation to the strategies used by the phytoplankton to take up iron in the oceans.
Authors:
Robert Sutak; Jan Slapeta; Mabel San Roman; Jean-Michel Camadro; Emmanuel Lesuisse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-19
Journal Detail:
Title:  Plant physiology     Volume:  154     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-05     Completed Date:  2011-01-03     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  991-1000     Citation Subset:  IM    
Affiliation:
Mitochondria Metals and Oxidative Stress, Institut Jacques Monod, Centre National de la Recherche Scientifique-Université Paris Diderot, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Alveolata / metabolism*
Biological Transport
Cell Wall / metabolism*
FMN Reductase / metabolism
Ferric Compounds / metabolism
Iron / metabolism*
Saccharomyces cerevisiae / metabolism
Siderophores / metabolism
Chemical
Reg. No./Substance:
0/Ferric Compounds; 0/Siderophores; 3522-50-7/ferric citrate; 7439-89-6/Iron; EC 1.5.1.29/FMN Reductase; EC 1.6.99.-/ferric citrate iron reductase
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