Document Detail


Non-protein-mediated transfer of phosphatidic acid between microsomal and mitochondrial membranes.
MedLine Citation:
PMID:  3341745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transfer of phosphatidic acid between rat liver microsomes loaded with [32P]-phosphatidic acid and rat liver mitochondria was studied in the absence of added lipid transfer proteins. It was found that during 1 h at 37 degrees C in the medium containing 100 mM KCl, 20-30% of phosphatidic acid but only 2.5% of phosphatidylcholine were transferred. This spontaneous transfer of phosphatidic acid remained the same after pretreatment of microsomes and mitochondria with 125 mM KCl or microsomes alone with 1 mM Tris, pH 8.6, procedures reported to remove adsorbed lipid transfer proteins. This transfer was insensitive to thiol-blocking reagents. The initial rate of this non-protein-mediated transfer of phosphatidic acid was virtually independent of the concentration of the acceptor membranes (mitochondria), thus indicating that it occurs by diffusion of the phospholipid through the aqueous phase rather than by membrane collision. About 80% of phosphatidic acid synthesized in the outer mitochondrial membrane was recovered in the inner membrane after a 1-h incubation, pointing to a high rate of the intermembrane transfer of this phospholipid within intact mitochondrion.
Authors:
J Barańska; L Wojtczak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  260     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-03-04     Completed Date:  1988-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-8     Citation Subset:  IM    
Affiliation:
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport, Active
Carrier Proteins / metabolism*
Intracellular Membranes / metabolism*
Microsomes, Liver / metabolism*
Mitochondria, Liver / metabolism*
Phosphatidic Acids / metabolism*
Protein Binding
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Phosphatidic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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