| Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose. | |
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MedLine Citation:
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PMID: 22461222 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). |
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Authors:
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Christian Brackmann; Jan-Olof Dahlberg; Nihal Engin Vrana; Caitriona Lally; Paul Gatenholm; Annika Enejder |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-28 |
Journal Detail:
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Title: Journal of biophotonics Volume: - ISSN: 1864-0648 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101318567 Medline TA: J Biophotonics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Molecular Microscopy, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Göteborg, Sweden. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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