Document Detail


Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose.
MedLine Citation:
PMID:  22461222     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Authors:
Christian Brackmann; Jan-Olof Dahlberg; Nihal Engin Vrana; Caitriona Lally; Paul Gatenholm; Annika Enejder
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-28
Journal Detail:
Title:  Journal of biophotonics     Volume:  -     ISSN:  1864-0648     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Molecular Microscopy, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Göteborg, Sweden.
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