| Non-lethal active caspase-3 expression in Bergmann glia of postnatal rat cerebellum. | |
| | |
MedLine Citation:
|
PMID: 16226814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Caspase-3, an apoptotic executor, has been shown in recent years to mediate non-lethal events like cellular proliferation and differentiation, primarily in studies related to non-neural tissue. In central nervous system development, the role of active caspase-3 is still unclear. We provide the first evidence for a potential new role of active (cleaved) caspase-3 in promoting differentiation of Bergmann glia. This study was predicated on the hypothesis that active caspase-3 is important for the differentiation of glia. We addressed the hypothesis through the following specific aims: (1) to establish the expression of active caspase-3 in glia; (2) to determine the developmental phenotype of the active caspase-3-expressing glia; and (3) to confirm that active caspase-3 expression is not mediating an apoptotic event. Through a temporal investigation from postnatal day 8 to 21, we observed that Bergmann glia express active caspase-3 without compromising their survival. Potential apoptotic fate of active caspase-3-positive Bergmann glia were ruled out based on immunohistochemical exclusion of phosphatidylserine exposure (Annexin V), DNA fragmentation (TUNEL), and DNA compaction (TOPRO-3). More than 90% of the active caspase-3-positive cells lacked colabeling for one of the apoptotic markers. Correlative studies using a proliferation marker Ki67 and a differentiation marker brain lipid-binding protein suggest that the expression of active caspase-3 was mostly associated with differentiating rather than proliferating Bergmann glia at all ages. Thus, this study supports the hypothesis that active caspase-3 may be regulating both differentiation of Bergmann glia by allowing the cells to exit the cell cycle and their morphogenesis. |
| | |
Authors:
|
Sowmini Oomman; Howard Strahlendorf; VelvetLee Finckbone; Jean Strahlendorf |
Related Documents
:
|
20814064 - Superoxide is a potential culprit of caspase-3 dependent endothelial cell death induced... 12934064 - Apoptosis caused by cathepsins does not require bid signaling in an in vivo model of pr... 8647264 - Ligation of cd40 rescues ramos-burkitt lymphoma b cells from calcium ionophore- and ant... 19913524 - Apoptosis induction in human leukemic cells by a novel protein bengalin, isolated from ... 18355244 - Differential and cell-type specific regulation of responses to toll-like receptor agoni... 20815784 - S-glutathiolation in life and death decisions of the cell. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-10-14 |
Journal Detail:
|
Title: Brain research. Developmental brain research Volume: 160 ISSN: 0165-3806 ISO Abbreviation: Brain Res. Dev. Brain Res. Publication Date: 2005 Dec |
Date Detail:
|
Created Date: 2005-12-02 Completed Date: 2006-02-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8908639 Medline TA: Brain Res Dev Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 130-45 Citation Subset: IM |
Affiliation:
|
Department of Physiology, Texas Tech University Health Sciences Center, Room 5A163, Lubbock, TX 79430, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Age Factors Animals Animals, Newborn Annexin A5 / metabolism Apoptosis / drug effects, physiology Calcium-Binding Protein, Vitamin D-Dependent / metabolism Caspase 3 Caspases / metabolism* Cell Count / methods Cerebellum / cytology*, growth & development, metabolism Fatty Acid-Binding Proteins / metabolism Gene Expression Regulation, Developmental / physiology* Immunohistochemistry / methods In Situ Nick-End Labeling / methods Ki-67 Antigen / metabolism Nerve Growth Factors / metabolism Nerve Tissue Proteins / metabolism Neuroglia / enzymology* Phosphopyruvate Hydratase / metabolism Quinolines / diagnostic use Rats Rats, Sprague-Dawley S100 Proteins / metabolism Statistics, Nonparametric Staurosporine / adverse effects Tubulin / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Annexin A5; 0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Ki-67 Antigen; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/Quinolines; 0/S-100 calcium-binding protein beta subunit; 0/S100 Proteins; 0/Tubulin; 0/calbindin; 62996-74-1/Staurosporine; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reconstitution of expression of H-2K region-encoded murine MHC class I glycoproteins in MHC class I-...
Next Document: Late outcomes after intervention of vein grafts in diabetics with abciximab use.