| Non-leaky vesiculation of large unilamellar vesicles (LUV) induced by plasma high density lipoproteins (HDL): detection by HPLC. | |
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MedLine Citation:
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PMID: 2730644 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interaction of large unilamellar phosphatidylcholine vesicles (LUV, 75nm) and plasma high density lipoproteins (HDL) resulted in a non-leaky vesiculation of LUV. This vesiculation was detected by a HPLC-system consisting of a combination of three TSK-gel columns (6000PW, 5000PW, 3000SW). With increasing incubation time liposomal [14C]PC, entrapped [3H]inulin, and apoprotein of HDL origin decreased. The decrease was accompanied by a formation of new particles, consisting of liposomal PC and apoprotein. These particles also enclosed [3H]inulin, reflecting a hydrophilic inner space. The formation of the particles reached a maximum after one day of incubation. Retention time was 21 minutes for LUV, 28 minutes for the new particles, and 36 minutes for HDL. In vesicles with membranes consisting of phosphatidylcholine and 30% cholesterol no interactions were observed. |
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Authors:
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U Tischler; D Rückert; R Schubert; H W Jaroni; K H Schmidt |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 160 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-07-10 Completed Date: 1989-07-10 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1219-26 Citation Subset: IM |
Affiliation:
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Department of Surgery, Chirurgische Klinik und Poliklinik, University of Tübingen, West Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Radioisotopes Cholesterol / pharmacology Chromatography, High Pressure Liquid Humans Inulin / metabolism Kinetics Lipoproteins, HDL / metabolism* Liposomes / metabolism* Phosphatidylcholines / metabolism Tritium |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Lipoproteins, HDL; 0/Liposomes; 0/Phosphatidylcholines; 10028-17-8/Tritium; 57-88-5/Cholesterol; 9005-80-5/Inulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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