Document Detail


Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus.
MedLine Citation:
PMID:  11862453     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The precise location of ribosomal RNA (rRNA) synthesis within the nucleolus is the subject of recent controversy; some investigators have detected nascent RNA in the dense fibrillar components (DFCs) while others have localized transcription to the fibrillar centers (FCs). We endeavored to resolve this controversy by applying a new technique for non-isotopic labeling of RNA and examined the synthesis and movement of non-isotopically labeled rRNA within the nucleolus. We found that rRNA is synthesized only in a restricted area of DFCs, also involving the boundary region with FCs. We traced a movement of RNA from transcription sites through DFCs to granular components. Our results indicate functional compartmentalization of DFCs with respect to the synthesis and processing of precursor rRNA. In situ mapping of the 5' leader sequence of the 5' external transcribed spacer together with transcription labeling indicated that transcription and the first steps in processing of precursor rRNA are spatially separated. Surprisingly, the results also pointed to a partially extended conformation of newly synthesized precursor rRNA transcripts.
Authors:
D Stanek; K Koberna; A Pliss; J Malínský; M Masata; J Vecerová; M C Risueño; I Raska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-11-09
Journal Detail:
Title:  Chromosoma     Volume:  110     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-02-25     Completed Date:  2002-05-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  460-70     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleolus / genetics,  metabolism*,  ultrastructure
Chromosomal Proteins, Non-Histone / metabolism
Female
Fluorescent Antibody Technique, Direct
Green Fluorescent Proteins
Hela Cells
Humans
In Situ Hybridization / methods*
Luminescent Proteins / analysis
Mice
Microscopy, Immunoelectron
RNA Precursors / biosynthesis,  genetics,  metabolism
RNA Processing, Post-Transcriptional*
RNA, Ribosomal / biosynthesis*,  genetics,  metabolism*
Rabbits
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/Luminescent Proteins; 0/RNA Precursors; 0/RNA, Ribosomal; 0/fibrillarin; 147336-22-9/Green Fluorescent Proteins

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