| Non-isotopic detection of nucleolar transcription in pre-implantation mouse embryos. | |
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MedLine Citation:
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PMID: 9606754 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleolar transcription was analysed in permeabilized pre-implantation mouse embryos at the four-cell, eight-cell, morula and early blastocyst stages using confocal microscopy to detect incorporated 5-bromouridine. The results demonstrated that the patterns of nucleolar transcription sites were common for all embryonic stages studied. They consisted most frequently of tightly associated groups of transcription foci similar to those encountered in somatic interphase cells. In addition, the nucleologenesis accompanying each cell cycle apparently gave rise to a different fluorescent pattern, that is to spatially separated fluorescent foci in the cells just after the resumption of rRNA synthesis. An immunoelectron microscopic analysis of the nucleolar transcription was also performed in the eight-cell embryos. A signal, usually consisting of clustered gold particles, was found specifically within nucleolar dense fibrillar components. This result was in agreement with established findings, which identify dense fibrillar component as the major site of nucleolar transcription in somatic cells. |
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Authors:
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K Koberna; V Landa; J Kanka; A Pliss; M Eltsov; D Stanĕk; I Raska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Reproduction, nutrition, development Volume: 38 ISSN: 0926-5287 ISO Abbreviation: Reprod. Nutr. Dev. Publication Date: 1998 Jan-Feb |
Date Detail:
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Created Date: 1998-07-01 Completed Date: 1998-07-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8913069 Medline TA: Reprod Nutr Dev Country: FRANCE |
Other Details:
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Languages: eng Pagination: 117-26 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Academy of Sciences of Czech Republic, Prague, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / metabolism*, ultrastructure Cell Nucleolus / metabolism* Embryonic Development* Female Mice Mice, Inbred C57BL Microscopy, Confocal Microscopy, Immunoelectron Morula / metabolism, ultrastructure Pregnancy Transcription, Genetic* Uridine / analogs & derivatives, metabolism |
| Chemical | |
Reg. No./Substance:
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58-96-8/Uridine; 957-75-5/5-bromouridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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