Document Detail


Non-involvement of the human monocarboxylic acid transporter 1 (MCT1) in the transport of phenolic acid.
MedLine Citation:
PMID:  16926505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phenolic acids such as p-coumaric acid and microbial metabolites of poorly absorbed polyphenols are absorbed by the monocarboxylic acid transporter (MCT)-mediated transport system which is identical to the fluorescein/H(+) cotransport system. We focus here on the physiological impact of MCT-mediated absorption and distribution. We examined whether MCT1, the best-characterized isoform found in almost all tissues, is involved in this MCT-mediated transport system. The induction of MCT1 expression in Caco-2 cells by a treatment with sodium butyrate (NaBut) did not increase the fluorescein permeability. Moreover, the transfection of Caco-2 cells with an expression vector encoding MCT1 caused no increase in either the permeability or uptake of fluorescein. Furthermore, in the MCT1-expressing oocytes, no increase of p-coumaric acid uptake was apparent, whereas the uptake of salicylic acid, a substrate of MCT1, nearly doubled. Our data therefore establish that MCT1 was not involved in the MCT-mediated transport of phenolic acids.
Authors:
Hirohito Watanabe; Takuya Yashiro; Yuichi Tohjo; Yutaka Konishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-23     Completed Date:  2006-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1928-33     Citation Subset:  IM    
Affiliation:
Department of Life Sciences, Faculty of Agriculture, Meiji University, Kanagawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Butyrates / pharmacology
Caco-2 Cells
Coumaric Acids / pharmacokinetics
Fluoresceins / chemistry,  pharmacokinetics
Humans
Hydroxybenzoic Acids / metabolism*
Monocarboxylic Acid Transporters / drug effects,  genetics,  metabolism*
Oocytes / drug effects
Permeability
Salicylic Acid
Symporters / drug effects,  genetics,  metabolism*
Transcription, Genetic / drug effects
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Butyrates; 0/Coumaric Acids; 0/Fluoresceins; 0/Hydroxybenzoic Acids; 0/Monocarboxylic Acid Transporters; 0/Symporters; 0/monocarboxylate transport protein 1; 29656-58-4/phenolic acid; 69-72-7/Salicylic Acid; 7400-08-0/4-coumaric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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