| Non-invasive imaging of transplanted human neural stem cells and ECM scaffold remodeling in the stroke-damaged rat brain by (19)F- and diffusion-MRI. | |
| | |
MedLine Citation:
|
PMID: 22244696 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Transplantation of human neural stem cells (hNSCs) is emerging as a viable treatment for stroke related brain injury. However, intraparenchymal grafts do not regenerate lost tissue, but rather integrate into the host parenchyma without significantly affecting the lesion cavity. Providing a structural support for the delivered cells appears important for cell based therapeutic approaches. The non-invasive monitoring of therapeutic methods would provide valuable information regarding therapeutic strategies but remains a challenge. Labeling transplanted cells with metal-based (1)H-magnetic resonance imaging (MRI) contrast agents affects the visualization of the lesion cavity. Herein, we demonstrate that a (19)F-MRI contrast agent can adequately monitor the distribution of transplanted cells, whilst allowing an evaluation of the lesion cavity and the formation of new tissue on (1)H-MRI scans. Twenty percent of cells labeled with the (19)F agent were of host origin, potentially reflecting the re-uptake of label from dead transplanted cells. Both T(2)- and diffusion-weighted MRI scans indicated that transplantation of hNSCs suspended in a gel form of a xenogeneic extracellular matrix (ECM) bioscaffold resulted in uniformly distributed cells throughout the lesion cavity. However, diffusion MRI indicated that the injected materials did not yet establish diffusion barriers (i.e. cellular network, fiber tracts) normally found within striatal tissue. The ECM bioscaffold therefore provides an important support to hNSCs for the creation of de novo tissue and multi-nuclei MRI represents an adept method for the visualization of some aspects of this process. However, significant developments of both the transplantation paradigm, as well as regenerative imaging, are required to successfully create new tissue in the lesion cavity and to monitor this process non-invasively. |
| | |
Authors:
|
Ellen Bible; Flavio Dell'acqua; Bhavana Solanky; Anthony Balducci; Peter M Crapo; Stephen F Badylak; Eric T Ahrens; Michel Modo |
Related Documents
:
|
8750316 - Anatomy of the medial temporal lobe. 20133346 - Periodic lateralized epileptiform discharges in neuropsychiatric lupus: association wit... 9551786 - Comparison of eeg, mri and pet in reading epilepsy: a case report. 8504786 - Identical morphology of the rolandic spike-and-wave complex in different clinical entit... 22381736 - Biomechanical correlates of symptomatic and asymptomatic neurophysiological impairment ... 2284936 - Partial trisomy 22 with dandy-walker malformation. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-12 |
Journal Detail:
|
Title: Biomaterials Volume: - ISSN: 1878-5905 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Kings College London, Institute of Psychiatry, Department of Neuroscience, London SE5 9NU, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electropora...
Next Document: The role of non-covalent interactions in anticancer drug loading and kinetic stability of polymeric ...