| Non-invasive, dynamic imaging of murine intestinal motility. | |
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MedLine Citation:
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PMID: 21624010 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: After intravenous (i.v.) administration, indocyanine green (ICG) is known to be secreted into bile from the liver via the biliary tracts, enabling fluorescent delineation of the intestine. In addition, ICG is a near-infrared (NIR) excitable fluorophore, capable of providing exogenous contrast for rapid NIR fluorescence imaging. We sought to quantify the intestinal motility using dynamic NIR fluorescence imaging after injection of ICG. METHODS: C57BL6 mice were dynamically imaged immediately before and up to 24h after i.v. and intradermal (i.d.) injection of 50 and 10μL of ICG, respectively. Necropsy was also performed 1h postinjection and the entire gastrointestinal tract was isolated and exposed for ex vivo fluorescence imaging. KEY RESULTS: The secretion of ICG-laden fluorescent bile into the duodenum was observed in vivo and confirmed in situ. Different patterns of the intestinal motility, such as peristaltic and segmental motions, were dynamically imaged in vivo. Our imaging data showed that the frequency of contractions ranged from 27 to 35cyclesmin(-1) and the propagation velocity of peristaltic waves ranged from 0.82±0.5 to 2.04±1.12cms(-1) . CONCLUSIONS & INFERENCES: Dynamic NIR fluorescence imaging with injection of ICG can provide a method for diagnostic motility testing for intestinal motility disorders or dysfunction and for potential evaluation of therapeutic agents. |
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Authors:
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S Kwon; E M Sevick-Muraca |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-05-31 |
Journal Detail:
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Title: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society Volume: 23 ISSN: 1365-2982 ISO Abbreviation: Neurogastroenterol. Motil. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-15 Completed Date: 2011-12-27 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 9432572 Medline TA: Neurogastroenterol Motil Country: England |
Other Details:
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Languages: eng Pagination: 881-e344 Citation Subset: IM |
Copyright Information:
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© 2011 Blackwell Publishing Ltd. |
Affiliation:
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Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, Texas 77030, USA. sunkuk.kwon@uth.tmc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Coloring Agents / metabolism* Female Gastrointestinal Motility / physiology* Gastrointestinal Tract / anatomy & histology*, physiology* Humans Indocyanine Green / metabolism* Mice Mice, Inbred C57BL Spectroscopy, Near-Infrared / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA128919/CA/NCI NIH HHS; R01 CA128919-04/CA/NCI NIH HHS; U54 CA136404/CA/NCI NIH HHS; U54 CA136404-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Coloring Agents; 3599-32-4/Indocyanine Green |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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