Document Detail


Non-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials.
MedLine Citation:
PMID:  23134271     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Investigating the safety of nanoparticles is essential for many fields of their applications, in particular for consumer products, food and medicines. The conventional dye and fluorescence based cytotoxicity assays are limited by the interference of such readouts with nanomaterials. This holds in particular when nanomaterials have been fluorescently labeled for other purposes, e.g. confocal microscopy. Moreover, most of these assays are invasive i.e. typically involve irreversible changes or destruction of cells and hence only allowing one endpoint measurement. Therefore, a non-invasive method for the detection of cytotoxicity was developed which is based on the automated online monitoring of the oxygen concentration in solution (SensorDish® Reader). Fluorescently-labeled silica nanoparticles with different sizes and surface modifications were used as model systems to explore this novel assay. Thereby, the SensorDish® Reader allows a life documentation of the cellular behavior and clarifies that size, time, concentration and surface modification of nanoparticles affect cellular viability.
Authors:
Andrea Neumeyer; Mirko Bukowski; Michael Veith; Claus-Michael Lehr; Nicole Daum
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Nanotoxicology     Volume:  -     ISSN:  1743-5404     ISO Abbreviation:  Nanotoxicology     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233132     Medline TA:  Nanotoxicology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Forschungszentrum Jülich, IBG-1, Jülich, Germany.
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