| Noninvasive assessment of murine pulmonary arterial pressure: validation and application to models of pulmonary hypertension. | |
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MedLine Citation:
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PMID: 20044514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Genetically modified mice offer the unique opportunity to gain insight into the pathophysiology of pulmonary arterial hypertension. In mice, right heart catheterization is the only available technique to measure right ventricular systolic pressure (RVSP). However, it is a terminal procedure and does not allow for serial measurements. Our objective was to validate a noninvasive technique to assess RVSP in mice. METHODS AND RESULTS: Right ventricle catheterization and echocardiography (30-MHz transducer) were simultaneously performed in mice with pulmonary hypertension induced acutely by infusion of a thromboxane analogue, U-46619, or chronically by lung-specific overexpression of interleukin-6. Pulmonary acceleration time (PAT) and ejection time (ET) were measured in the parasternal short-axis view by pulsed-wave Doppler of pulmonary artery flow. Infusion of U-46619 acutely increased RVSP, shortened PAT, and decreased PAT/ET. The pulmonary flow pattern changed from symmetrical at baseline to asymmetrical at higher RVSPs. In wild-type and interleukin-6-overexpressing mice, the PAT correlated linearly with RVSP (r(2)=-0.67, P<0.0001), as did PAT/ET (r(2)=-0.76, P<0.0001). Sensitivity and specificity for detecting high RVSP (>32 mm Hg) were 100% (7/7) and 86% (6/7), respectively, for both indices (cutoff values: PAT, <21 ms; PAT/ET, <39%). Intraobserver and interobserver variability of PAT and PAT/ET were <6%. CONCLUSIONS: Right ventricular systolic pressure can be estimated noninvasively in mice. Echocardiography is able to detect acute and chronic increases in RVSP with high sensitivity and specificity as well as to assess the effects of treatment on RVSP. This noninvasive technique may permit the characterization of the evolution of pulmonary arterial hypertension in genetically modified mice. |
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Authors:
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Hélène B Thibault; Baptiste Kurtz; Michael J Raher; Rahamthulla S Shaik; Aaron Waxman; Geneviève Derumeaux; Elkan F Halpern; Kenneth D Bloch; Marielle Scherrer-Crosbie |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Studies Date: 2009-12-31 |
Journal Detail:
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Title: Circulation. Cardiovascular imaging Volume: 3 ISSN: 1942-0080 ISO Abbreviation: Circ Cardiovasc Imaging Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-17 Completed Date: 2010-05-07 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 101479935 Medline TA: Circ Cardiovasc Imaging Country: United States |
Other Details:
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Languages: eng Pagination: 157-63 Citation Subset: IM |
Affiliation:
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Cardiac Ultrasound Laboratory, Cardiology Division of Department of Medicine, Cardiovascular Research Center, Anesthesia Center for Critical Care Research, Massachusetts General Hospital, Boston, MA 02114, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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pharmacology Analysis of Variance Animals Disease Models, Animal Echocardiography, Doppler / methods* Heart Catheterization Hypertension, Pulmonary / physiopathology*, ultrasonography* Image Processing, Computer-Assisted Interleukin-6 / pharmacology Mice Mice, Inbred C57BL Mice, Transgenic Pulmonary Artery / physiopathology* Regression Analysis Sensitivity and Specificity |
| Grant Support | |
ID/Acronym/Agency:
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1S10RR022586-01A1/RR/NCRR NIH HHS; R01 HL074352-06/HL/NHLBI NIH HHS; R01 HL074352-07/HL/NHLBI NIH HHS; S10 RR022586-01A1/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid |
| Comments/Corrections | |
Comment In:
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Circ Cardiovasc Imaging. 2010 Mar;3(2):132-3
[PMID:
20233861
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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