Document Detail


Non-genomic, direct modulatory effect of 17β-estradiol, progesterone and their synthetic derivatives on the activity of human erythrocyte CuZn superoxide dismutase.
MedLine Citation:
PMID:  23297859     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract This study aimed to evaluate whether natural or synthetic steroid hormones could directly modulate the activity of the different superoxide dismutase (SOD) isoforms found in human blood fractions without changing enzyme expression. Enzyme samples of human erythrocytes, the human platelet-rich plasma fraction (PRP) or isolated CuZnSOD, which was purified from human erythrocytes were pre-incubated with natural steroids (17β-estradiol 17-acetate and progesterone) and their synthetic derivatives (β-estradiol 3-benzoate and medroxyprogesterone 17-acetate). Then, CuZn and MnSOD activities were measured using the xanthine/xanthine oxidase/nitroblue tetrazolium method. Hormones had no effect on MnSOD activity from the PRP, but we show for the first time that natural and synthetic steroid hormones have a direct, bell-shaped effect on the activity of CuZnSOD from both male and female human erythrocytes. Low (physiological) hormone concentrations caused a dose-dependent increase in enzyme activity, which disappeared at higher hormone concentrations. In addition, the combination of synthetic and natural estrogens and progestins had a synergistic stimulatory effect on the activity of CuZnSOD from human erythrocytes. The molecular interaction between CuZnSOD and steroid hormones was preliminarily studied. Natural hormones did not change the electrophoretic mobility of SOD under denaturing conditions, but they did increase the absorption spectra of SOD in the 230-290 nm range. These data suggest that hormone-mediated modulation of CuZnSOD is related to subtle changes in protein conformation, possibly related to Trp and Phe residues. We propose that this effect may account for the physiological regulation of enzyme activity during conditions where steroid hormones undergo alterations as the ovulatory cycle.
Authors:
T C Unfer; L H Maurer; D M Kemerich; C Figueiredo; M M F Duarte; D P Gelain; J C F Moreira; T Emanuelli
Related Documents :
23594729 - Comparison of activity indexes for recognizing enzyme mutants of higher activity with u...
23698049 - Interactive effect of salicylic acid on some physiological features and antioxidant enz...
24031819 - Screening of actinomycetes from earthworm castings for their antimicrobial activity and...
24684619 - A single molecule perspective on the functional diversity of in vitro-evolved beta-gluc...
23201419 - Identification of glyoxalase 1 polymorphisms associated with enzyme activity.
15177289 - Functional expression, purification, and characterization of the extra stable human pla...
2995339 - Enzymatic properties of separated isozymes of the na,k-atpase. substrate affinities, ki...
17920159 - Identification of enzyme activity that conjugates indole-3-acetic acid to aspartate in ...
15588679 - The composition, geographical variation and antimicrobial activity of lippia javanica (...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Free radical research     Volume:  -     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Modulatory mechanisms of enterocyte apoptosis by viral, bacterial and parasitic pathogens.
Next Document:  Cognitive impairment and cortical reorganization in children with benign epilepsy with centrotempora...