Document Detail

Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro.
MedLine Citation:
PMID:  16183866     Owner:  NLM     Status:  MEDLINE    
In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) of high-yielding dairy cows were determined during the period of negative energy balance (NEB) early post partum. NEFA were then added during in vitro maturation at concentrations measured previously in FF to evaluate their effect on the oocyte's developmental competence. At 16 and 44 days post partum, FF of the dominant follicle and blood were collected from nine high-yielding dairy cows. Samples were analysed for NEFA concentration and composition. NEFA concentrations in FF (0.2-0.6 mmol/l) during NEB remained +/- 40% lower compared with serum (0.4-1.2 mmol/l). The NEFA composition differed significantly between serum and FF with oleic acid (OA), palmitic acid (PA) and stearic acid (SA) being the predominant fatty acids in FF. Based on these results, 5115 oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of 0.200 mmol/l OA, 0.133 mmol/l PA or 0.067 mmol/l SA dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in SOF medium. Addition of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield. More (late) apoptotic cumulus cells were observed in cumulus-oocyte complexes matured in the presence of SA or PA. Ethanol or OA had no effect. These in vitro results suggest that NEB may hamper fertility of high-yielding dairy cows through increased NEFA concentrations in FF affecting oocyte quality.
J L M R Leroy; T Vanholder; B Mateusen; A Christophe; G Opsomer; A de Kruif; G Genicot; A Van Soom
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  130     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-26     Completed Date:  2006-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  485-95     Citation Subset:  IM    
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
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MeSH Terms
Blastocyst / metabolism
Cattle / metabolism*
Cells, Cultured
Cleavage Stage, Ovum / metabolism
Culture Media, Serum-Free
Energy Metabolism*
Fatty Acids, Nonesterified / analysis*,  metabolism
Fertilization in Vitro
Follicular Fluid / chemistry*,  metabolism
Milk Ejection
Oleic Acid / pharmacology
Oocytes / metabolism,  physiology*
Oogenesis / physiology*
Palmitic Acid / pharmacology
Postpartum Period / metabolism
Stearic Acids / pharmacology
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Fatty Acids, Nonesterified; 0/Stearic Acids; 112-80-1/Oleic Acid; 57-10-3/Palmitic Acid; 57-11-4/stearic acid; 64-17-5/Ethanol

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