Document Detail


Non-enzymatic interactions of glyoxylate with lysine, arginine, and glucosamine: a study of advanced non-enzymatic glycation like compounds.
MedLine Citation:
PMID:  16970975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glyoxylate is a 2 carbon aldo acid that is formed in hepatic tissue from glycolate. Once formed, the molecule can be converted to glycine by alanine-glyoxylate aminotransferase (AGAT). In defects of AGAT, glyoxylate is transformed to oxalate, resulting in high levels of oxalate in the body. The objective of this study was 2-fold. First, it was to determine, if akin to D-glucose, D-fructose or DL-glyceraldehyde, glyoxylate was susceptible to non-enzymatic attack by amino containing molecules such as lysine, arginine or glucosamine. Second, if by virtue of its molecular structure and size, glyoxylate was as reactive a reagent in non-enzymatic reactions as DL-glyceraldehyde; i.e., a glycose that we previously demonstrated to be a more effective glycating agent than D-glucose or D-fructose. Using capillary electrophoresis (CE), high performance liquid chromatography and UV and fluorescence spectroscopy, glyoxylate was found to be a highly reactive precursor of advanced glycation like end products (AGLEs) and a more effective promoter of non-enzymatic end products than D-glucose, D-fructose or DL-glyceraldehyde.
Authors:
Udayan Dutta; Menashi A Cohenford; Madhumita Guha; Joel A Dain
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-09-12
Journal Detail:
Title:  Bioorganic chemistry     Volume:  35     ISSN:  0045-2068     ISO Abbreviation:  Bioorg. Chem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-23     Completed Date:  2007-04-16     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  1303703     Medline TA:  Bioorg Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  11-24     Citation Subset:  IM    
Affiliation:
Department of Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
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MeSH Terms
Descriptor/Qualifier:
Arginine / chemistry*
Chromatography, High Pressure Liquid
Electrophoresis, Capillary
Fructose / chemistry
Glucosamine / chemistry*
Glucose / chemistry
Glyceraldehyde / chemistry
Glycosylation End Products, Advanced / chemistry*
Glyoxylates / chemistry*
Lysine / analogs & derivatives,  chemistry*
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Grant Support
ID/Acronym/Agency:
P20 RR016457/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Glycosylation End Products, Advanced; 0/Glyoxylates; 298-12-4/glyoxylic acid; 30237-26-4/Fructose; 3416-24-8/Glucosamine; 367-47-5/Glyceraldehyde; 50-99-7/Glucose; 56-87-1/Lysine; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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