Document Detail


Non-destructive assessment of polycyclic aromatic hydrocarbon (PAH) exposure by fluorimetric analysis of crab urine.
MedLine Citation:
PMID:  18845307     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The detection of urinary polycyclic aromatic hydrocarbon (PAH) metabolites by fluorescence spectrophotometry is particularly effective as a practical means to assess PAH exposure in decapod crabs. However, the practical application of this technique has thus far only been tested for the European shore crab (Carcinus maenas) and only a few field studies have been conducted in heavily polluted areas. The present study evaluated the adaptability of this method as a rapid, cost-effective and non-destructive biomonitoring tool for the New Zealand crab species, Macrophthalmus hirtipes (stalk-eyed mud crab). A field gradient could be detected among the sites and different input sources of PAH contamination could be discerned through the differentiation of pyrogenic and petrogenic PAH signatures. The present study shows that the fluorescence screening method is sensitive to relatively low levels of PAH contamination and more broadly applicable to smaller crab species than C. maenas, for which the technique was developed.
Authors:
Samuel Koenig; Candida Savage; Jonathan P Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-08
Journal Detail:
Title:  Marine pollution bulletin     Volume:  56     ISSN:  0025-326X     ISO Abbreviation:  Mar. Pollut. Bull.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-02     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0260231     Medline TA:  Mar Pollut Bull     Country:  England    
Other Details:
Languages:  eng     Pagination:  2003-8     Citation Subset:  IM    
Affiliation:
Department of Marine Science, University of Otago, 310 Castle Street, Dunedin 9016, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brachyura / chemistry*,  metabolism*
Fluorometry / methods*
Male
Polycyclic Hydrocarbons, Aromatic / analysis*
Urine / chemistry*
Water Pollutants, Chemical / chemistry
Chemical
Reg. No./Substance:
0/Polycyclic Hydrocarbons, Aromatic; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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