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A Non-damaging Method to Analyze the Configuration and Dynamics of Nitrotyrosines in Proteins.
MedLine Citation:
PMID:  22378295     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Often, deregulation of protein activity and turnover by tyrosine nitration drives cells toward pathogenesis. Hence, understanding how the nitration of a protein affects both its function and stability is of outstanding interest. Nowadays, most of the in vitro analyses of nitrated proteins rely on chemical treatment of native proteins with an excess of a chemical reagent. One such reagent, peroxynitrite, stands out for its biological relevance. However, given the excess of the nitrating reagent, the resulting in vitro modification could differ from the physiological nitration. Here, we determine unequivocally the configuration of distinct nitrated-tyrosine rings in single-tyrosine mutants of cytochrome c. We aimed to confirm the nitration position by a non-destructive method. Thus, we have resorted to (1) H-(15) N heteronuclear single quantum coherence(HSQC) spectra to identify the (3) J(NH) correlation between a (15) N-tagged nitro group and the adjacent aromatic proton. Once the chemical shift of this proton was determined, we compared the (1) H-(13) C HSQC spectra of untreated and nitrated samples. All tyrosines were nitrated at ε positions, in agreement to previous analysis by indirect techniques. Notably, the various nitrotyrosine residues show a different dynamic behaviour that is consistent with molecular dynamics computations.
Authors:
Irene Díaz-Moreno; Pedro M Nieto; Rebecca Del Conte; Margarida Gairí; José M García-Heredia; Miguel A De la Rosa; Antonio Díaz-Quintana
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-29
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  -     ISSN:  1521-3765     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla-C.S.I.C. Avda. Américo Vespucio, 49, 41092 Sevilla (Spain), Fax: (+34) 954460065. idiazmoreno@us.es.
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