Document Detail

Non-coordinate synthesis of RNA polymerase beta beta' subunits in a temperature-sensitive beta'-subunit mutant of Escherichia coli.
MedLine Citation:
PMID:  6759869     Owner:  NLM     Status:  MEDLINE    
On exposure to high temperature of a temperature-sensitive RNA polymerase beta' subunit (rpoC92) mutant of Escherichia coli, selective reduction was observed in the rate of synthesis of a group of proteins including RNA polymerase beta' subunit. The finding that the synthesis of beta' subunit but not beta subunit was specifically repressed in this mutant grown at non-permissive temperature indicates that the functionally intact RNA polymerase is required for the synthesis of beta beta' subunits be coordinated. In addition, the assembly of newly synthesized RNA polymerase subunits was inefficient in this mutant at the steps where altered beta' subunit was involved, and the unassembled enzyme subunits were rapidly and preferentially degraded. During recovery to non-restricted growth, the synthesis of both beta and beta' subunits was transiently enhanced in parallel leading to recovery of the intracellular concentration of functional RNA polymerase.
N Nakada; K Yoshinaga; A Ishihama; H Nagasawa-Fujimori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  188     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1982  
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  173-8     Citation Subset:  IM    
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MeSH Terms
DNA-Directed RNA Polymerases / biosynthesis,  genetics*,  metabolism
Escherichia coli / genetics*
Gene Expression Regulation
Macromolecular Substances
Molecular Weight
Reg. No./Substance:
0/Macromolecular Substances; EC RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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