|Non-cell-autonomous RNA interference in mammalian cells: Implications for in vivo cell-based RNAi delivery.|
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|PMID: 22205888 Owner: NLM Status: PubMed-not-MEDLINE|
|RNA interference (RNAi) is a post-transcriptional pathway in which double-stranded RNA (dsRNA) triggers the degradation of complementary mRNA in the cytoplasm of eukaryotic cells. In plants and in some animals, including Caenorhabditis elegans, initiation of RNAi in one cell can lead to sequence-specific RNA silencing in another cell, a phenomenon referred to as non-cell-autonomous RNAi. Until recently, this phenomenon had not been observed in mammalian cells. Here, we review emerging data demonstrating that non-cell-autonomous RNAi occurs in cultured mammalian cells. We discuss possible mechanisms for the transfer of RNAi between mammalian cells and highlight the implications of this phenomenon for the development of in vivo cell-based RNAi delivery.|
|Hannah C Cohen; May P Xiong|
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|Type: Journal Article Date: 2011-12-01|
|Title: Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research Volume: 7 ISSN: 1747-0854 ISO Abbreviation: J RNAi Gene Silencing Publication Date: 2011|
|Created Date: 2011-12-29 Completed Date: 2012-10-02 Revised Date: 2013-05-29|
Medline Journal Info:
|Nlm Unique ID: 101256512 Medline TA: J RNAi Gene Silencing Country: England|
|Languages: eng Pagination: 456-63 Citation Subset: -|
|Pharmaceutical Sciences Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Journal ID (nlm-ta): J RNAi Gene Silencing
Journal ID (publisher-id): jrgs
Publisher: Library Publishing Media
Received Day: 18 Month: 10 Year: 2011
Revision Received Day: 01 Month: 11 Year: 2011
Accepted Day: 09 Month: 11 Year: 2011
collection publication date: Year: 2011
Electronic publication date: Day: 01 Month: 12 Year: 2011
Volume: 7First Page: 456 Last Page: 463
PubMed Id: 22205888
|Non-cell-autonomous RNA interference in mammalian cells: Implications for in vivo cell-based RNAi delivery|
|Hannah C Cohen|
|May P Xiong*|
|Pharmaceutical Sciences Division, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705, USA
|*Correspondence to: May Xiong, firstname.lastname@example.org Tel: +608 890 0699, Fax: +608 262 5345
RNA interference (RNAi) is a post-transcriptional pathway in which double-stranded RNA (dsRNA) triggers the degradation of complementary mRNA in the cytoplasm of eukaryotic cells (Fire et al, 1998). In this pathway, the ribonuclease III enzyme Dicer cleaves long dsRNA (Bernstein et al, 2001) into ∼21–25-nucleotide (nt) dsRNA duplexes (Hammond et al, 2000; Zamore et al, 2000) referred to as short hairpin RNAs (shRNAs), microRNAs (miRNAs) or short interfering RNAs (siRNAs). (See Figure 1 for more detail regarding when each term is used). The resulting dsRNA duplex is incorporated into the RNA-induced silencing complex (RISC) (Hammond et al, 2000), where helicase enzymes unwind the duplex and the catalytic protein Argonaute 2 (Ago2) cleaves the sense strand of RNA (Matranga et al, 2005). Guided by the antisense strand of RNA (Martinez et al, 2002), the activated RISC targets complementary mRNAs in the cytoplasm for translational repression, mRNA degradation, or cleavage by Ago2 (Liu et al, 2004; Rand et al, 2004; reviewed in Fabian et al, 2010).
In plants and in some animals, including Caenorhabditis elegans, RNAi can spread intercellularly (reviewed in Mlotshwa et al, 2002; Voinnet, 2005; Jose and Hunter, 2007; Dinger et al, 2008; Kalantidis et al, 2008; Chitwood and Timmermans, 2010), a phenomenon referred to as non-cell-autonomous RNAi. In plants, RNAi spreads locally from cell-to-cell through plasmodesmata (Himber et al, 2003), which connect the cytosols of adjacent plant cells. RNAi also spreads systemically through the phloem system of the plant (Voinnet et al, 1998; Himber et al, 2003). In C. elegans, injection of dsRNA into the pseudocoelomic body cavity or gonad triggers RNAi in somatic tissues (Fire et al, 1998). Additionally, feeding C. elegans with dsRNA or dsRNA-expressing bacteria induces systemic RNAi (Timmons and Fire, 1998).
Despite numerous reports of non-cell-autonomous RNAi in plants and in some animals, until recently, this phenomenon had not been observed in mammalian cells. Here, we review emerging data demonstrating the transfer of RNAi between cultured mammalian cells by both cell contact-independent and cell contact-dependent transfer mechanisms. The existence of non-cell-autonomous RNAi in mammalian cells has important implications for the development of in vivo cell-based RNAi delivery.
Microvesicles (MVs) are plasma membrane fragments varying in size (0.1–1μm in diameter), shape, and composition that are shed from a variety of healthy or damaged cells during plasma membrane blebbing. MVs are thought to mediate intercellular communication by delivering proteins, RNAs, and other cellular cargos from one cell to another (reviewed in Ratajczak et al, 2006b; Cocucci et al, 2009). In agreement, Ratajczak et al (2006a) showed that MVs derived from embryonic stem cells (ESCMVs) delivered embryonic stem cell-derived mRNA into hematopoietic progenitor cells (HPCs), resulting in target protein production and reprogramming of the HPCs. Furthermore, MVs derived from a variety of cell types contain miRNAs (Hunter et al, 2008; Yuan et al, 2009; Collino et al, 2010), suggesting that MVs may shuttle miRNAs between cells. Interestingly, Collino et al (2010) detected certain miRNAs in MVs, but not in their parental cells, suggesting that such miRNAs are selectively packaged in MVs for non-cell-autonomous RNAi.
In further supporting the hypothesis that MVs shuttle miRNAs between cells, Yuan et al (2009) showed that ESCMVs fused with co-incubated mouse embryonic fibroblasts to deliver miRNAs. Additionally, Collino et al (2010) demonstrated that miRNA-containing MVs released from donor human bone marrow-derived mesenchymal stem cells (MSCs) delivered miRNAs into co-incubated recipient cells, resulting in target-specific reduction in protein levels. These data demonstrate that certain cell types release MVs that can shuttle functional miRNAs between cells (Figure 2A).
Also involved in intercellular communication, exosomes are small, 30–100nm in diameter, membrane-bound intraluminal vesicles (ILVs) that are secreted by most cells. Formed during the endolysosomal pathway by the invagination of the limiting endosomal membrane in multivesicular bodies (MVBs), exosomes are released into the extracellular space when MVBs fuse with the plasma membrane. Exosomes contain cytosolic and plasma membrane proteins and express cell recognition molecules that allow for selective cellular uptake (reviewed in Théry et al, 2002; Simons and Raposo, 2009; Mincheva-Nilsson and Baranov, 2010). Similarly, secretory exosomes containing RNAs and proteins may be a major mode of communication within the nervous system (reviewed in Smalheiser, 2007). Exosomes secreted from a variety of cell types contain miRNAs (Valadi et al, 2007; Skog et al, 2008; Taylor and Gercel-Taylor, 2008; Luo et al, 2009; Kosaka et al, 2010; Michael et al, 2010; Ohshima et al, 2010; Pegtel et al, 2010; Zomer et al, 2010; DeIuliis et al, 2011; Levänen et al, 2011; Mittelbrunn et al, 2011), leading to the name "exosomal shuttle RNA" (Lotvall and Valadi, 2007; Valadi et al, 2007). In fact, certain miRNAs exist in higher levels in exosomes than in their parental cells (Valadi et al, 2007; Ohshima et al, 2010; DeIuliis et al, 2011; Mittelbrunn et al, 2011), suggesting that such miRNAs are selectively packaged into MVBs and secreted as exosomes for non-cell-autonomous RNAi.
Pegtel et al (2010) has provided further evidence to support the hypothesis that exosomes shuttle miRNAs between cells by demonstrating that Epstein-Barr virus (EBV)-infected B cells secrete exosomes containing EBV-encoded miRNAs, which knocked down EBV target genes in co-incubated monocyte-derived dendritic cells. Similarly, Kosaka et al (2010) showed that cultured HEK293 human embryonic kidney cells and COS-7 African green monkey kidney fibroblast-like cells secrete miRNA-containing exosomes, which were internalized in recipient cells leading to sequence-specific RNA silencing. These data demonstrate that certain cell types release exosomes that can shuttle functional miRNAs between cells (Figure 2B).
In addition to MVs and exosomes, apoptotic bodies – a form of MVs released during programmed cell death – may transport miRNAs between cells (Figure 2C). Zernecke et al (2009) demonstrated that apoptotic bodies isolated from cultured endothelial cells could deliver miRNA-126 into recipient vascular cells, inducing the production of chemokine CXC motif ligand 12 (CXCL12). Alternatively, miRNAs were shown to exist extracellularly in human plasma as vesicle-free Ago2 complexes (Arroyo et al, 2011). Because Ago2 is the catalytic component of RISC (Liu et al, 2004; Rand et al, 2004), these miRNA-Ago2 complexes could be responsible for inducing RNAi in recipient cells (Figure 2D).
SID-1 (systemic RNAi-defective-1) is a protein channel that is necessary for the import of dsRNA into most cells in C. elegans (Winston et al, 2002; Feinberg and Hunter, 2003). Overexpression of the mammalian homologue of SID-1 (i.e., SIDT-1) was shown to increase internalization of siRNA and RNAi in mammalian cells soaked in siRNA-containing medium (Duxbury et al, 2005; Tsang et al, 2007) (Figure 2E). However, at endogenous levels, SIDT-1 expression appeared to be insufficient for siRNA uptake into mammalian cells (Tsang et al, 2007).
Tunneling nanotubes (TNTs) were first identified in cultured rat pheochromocytoma PC12 cells as intercellular structures with diameters of 50–200nm and lengths of up to several cell diameters (Rustom et al, 2004). They were shown to hover between cells, transferring membrane components and organelles between the cytosols of adjoining cells (Rustom et al, 2004). TNTs with a variety of diameters, lengths, and compositions have since been identified in vitro in numerous cell types (reviewed in Gerdes and Carvalho, 2008; Gurke et al, 2008) and in vivo between bone marrow-derived MHC class II-positive cells in the corneal stroma of mice (Chinnery et al, 2008).
Two types of TNTs have been identified in cultured human macrophages: TNTs less than 0.7μm in diameter that contain F-actin, and TNTs greater than 0.7μm in diameter that contain F-actin and microtubules (Önfelt et al, 2006). Bidirectional transfer of mitochondria and intracellular vesicles (including late endosomes and lysosomes) can occur in the latter variety of TNTs (Önfelt et al, 2006), suggesting that TNTs could mediate the transfer of miRNA-containing endosomal vesicles between cells (Figure 2F). In agreement, Belting and Wittrup (2008) reviewed TNTs as a potential mechanism for the intercellular transfer of genetic material, due in part to their ability to transfer endosomal vesicles between cells. Additionally, TNTs are remarkably similar to plasmodesmata (reviewed in Rustom, 2009), which are involved in the transfer of siRNAs between plant cells. In fact, donor human MSCs were shown to infuse siRNAs into co-cultured recipient neural progenitor cells (Mitchell et al, 2011; Olson et al, 2011) through TNTs and other mechanisms (Nolta JA, personal communication).
Gap junctions are intercellular channels that span the plasma membranes of adjoining cells, connecting the cytosols and allowing for cell-to-cell communication (reviewed in Bruzzone et al, 1996). In a gap junction, each of two adjacent cells contains a connexon, which is a hemichannel composed of six connexin (Cx) proteins. Gap junctions form from two identical connexons (homotypic) or two different connexons (heterotypic). Most tissues express more than one type of connexin and connexins often have distinct tissue and cellular distributions. Gap junction intercellular communication (GJIC) allows water-soluble molecules (e.g., ions, second messengers, and small metabolites) to diffuse between cells (reviewed in Bruzzone et al, 1996). Emerging evidence suggests that GJIC also mediates the transfer of RNAi between cells.
Valiunas et al (2005) demonstrated that there was transfer of RNAi targeting DNA polymerase β from donor normal rat kidney (NRK) cells stably expressing shRNA against DNA polymerase β to co-cultured recipient NRK cells (Valiunas et al, 2005). NRK cells express C×43 (Hand et al, 2002); however, repeated experiments using donor and recipient cells that expressed C×26 and C×32 but not C×43, or that were connexin-deficient, did not show knockdown of DNA polymerase β in recipient cells (Valiunas et al, 2005).
In similar co-culture experiments, Wolvetang et al (2007) demonstrated a gap junction (C×43 and/or C×45)-mediated transfer of RNAi targeting green fluorescent protein (GFP) from donor human embryonic stem cells (hESCs) stably expressing shRNA against GFP to co-cultured recipient hESCs stably expressing GFP. Likewise, Kizana et al (2009) observed the transfer of RNAi targeting enhanced GFP (eGFP) from donor neonatal rat ventricular myocytes (NRVMs) stably expressing shRNA against eGFP to co-cultured recipient NRVMs stably expressing eGFP. NRVMs express C×43 (Kizana et al, 2007); however, there was no change in eGFP levels when the NRVMs expressed a C×43 dominant-negative mutant, suggesting that the reduction in eGFP was dependent on C×43 gap junctions (Kizana et al, 2009). Lastly, Lim et al (2011) reported that miRNAs against CXCL12 were transferred from donor bone marrow stromal cells to co-cultured recipient breast cancer cells through gap junctions composed of C×43, leading to reduced expression of CXCL12 in the recipient cells. Together, these studies demonstrate that RNAi transfers between cells through gap junctions composed of certain connexins, including C×43 (Figure 2G).
An immunological synapse (IS) is a junction that forms at the interface of a T cell and an antigen-presenting cell (APC), allowing for cell-to-cell interactions to modulate the immune response (reviewed in Rodríguez-Fernández et al, 2010). Mittelbrunn et al (2011) demonstrated that miRNA-containing exosomes transferred from T cells to APCs upon antigen-induced IS formation, resulting in sequence-specific RNA silencing in the recipient APCs. The T cell MVBs polarized towards the IS, which enhanced the secretion of miRNA-containing exosomes (Mittelbrunn et al, 2011). These data suggest that RNAi may transfer between immune cells through ISs (Figure 2H).
Different methods of introducing the RNAi agent into the donor cells may impact the transfer of RNAi between cells. For example, a common method of transfecting cells with siRNA involves complexing the siRNA with a cationic lipid carrier to create siRNA-lipoplexes. Recent data suggest that a majority of siRNA-lipoplexes introduced into cells persist in endolysosomes (Lu et al, 2009). Because endolysosomal trafficking may be involved in intercellular transfer of RNAi (reviewed in Gibbings and Voinnet, 2010), siRNA-lipoplexes within endolysomes may be more likely to transfer between cells than siRNA introduced into cells by a different method.
The method of introducing the RNAi agent into the donor cells may also impact the potency of RNA silencing in the recipient cells. Assuming that the donor cells remain viable at the target site, donor cells stably expressing shRNA or miRNA may induce more potent RNA silencing in the recipient cells than donor cells transiently expressing siRNA.
Increasing the ratio of siRNA-expressing donor cells to recipient cells in co-culture increased target gene knockdown in recipient cells (Valiunas et al, 2005; Wolvetang et al, 2007), suggesting that a higher ratio of donor to recipient cells results in more RNA silencing signals entering the recipient cells. By increasing the ratio of donor to recipient cells, we expect to eventually reach a maximum percentage of target gene knockdown in recipient cells (Figure 3). This maximum would depend on a variety of factors including the rate of target mRNA turnover in recipient cells, the number of RNA silencing signals transferred by each donor cell, and the potency of each RNA silencing signal. Additionally, in cell contact-dependent RNAi transfer, limited points of contact between the donor and recipient cells may increase the time of co-culture required to reach the maximum target gene knockdown in recipient cells or may reduce the maximum altogether.
While the transfer of RNA silencing signals from donor to recipient cells may lessen the potency of RNAi in the donor cells, we predict that the transfer would not eliminate RNAi in the donor cells. Each activated RISC undergoes multiple rounds of RNA silencing (Hutvágner and Zamore, 2002), suggesting that potent RNA silencing could occur in the donor cells despite the transfer of some RNA silencing signals to the recipient cells. Additionally, an RNA-dependent RNA polymerase (RdRP) has recently been identified in mammalian cells (Maida et al, 2009).
RdRPs are primed by existing siRNAs to produce more dsRNAs, thereby amplifying RNA silencing (Sijen et al, 2001; reviewed in Nishikura, 2001). Thus, the transfer of RNA silencing signals from donor to recipient cells would not necessarily eliminate RNAi in the donor cells. Unfortunately, limited understanding of the molecular nature of the transferred RNA silencing signal makes it difficult to predict how RdRPs or the multi-turnover nature of RISC impacts the transfer of RNAi between mammalian cells.
In plants, the RNA silencing signal that spreads short distances (10–15 cells) from cell to cell through plasmodesmata is a 21-nt siRNA duplex (Himber et al, 2003; Dunoyer et al, 2005; Dunoyer et al, 2010; reviewed in Chitwood and Timmermans, 2010). However, the molecular nature of the RNA silencing signal that spreads systemically in plants and in C. elegans remains unclear (reviewed in Mlotshwa et al, 2002), as does the molecular nature of the transferred RNA silencing signal in mammalian cells.
It is unlikely that long dsRNA is the RNA silencing signal that transfers between mammalian cells, as dsRNAs longer than 30nt activate the interferon system (Minks et al, 1979; Manche et al, 1992). In agreement, Valiunas et al (2005), Wolvetang et al (2007), and Kizana et al (2009) hypothesized that Dicer-processed shRNA was the RNA silencing signal transferred between mammalian cells through gap junctions. Valiunas et al (2005) demonstrated that oligonucleotides (morphilinos) simulating siRNAs, with molecular weights of ∼2–4kDa, minor diameters of 1.0–1.1nm, and lengths of 7.6nm could diffuse between cells through C×43 gap junctions. The oligonucleotide permeation through the C×43 gap junctions decreased as the length of the oligonucleotides increased, which was expected as the minor diameter of the oligonucleotides was close to the pore diameter of the gap junctions (∼1.0–1.5nm) (Valiunas et al, 2005). A hybridized, double-stranded 12-mer oligonucleotide had significantly decreased permeation through the C×43 gap junctions when compared with a single-stranded 12-mer oligonucleotide, suggesting that single-stranded siRNAs are more likely to transfer through C×43 gap junctions than hybridized siRNAs (Valiunas et al, 2005). Additionally, Kizana et al (2009) determined that, compared with shRNA-expressing donor cells, co-cultured recipient cells contained 25% of the copy number of the antisense strand of shRNA, suggesting that the antisense strand of shRNA, or possibly the shRNA duplex, transfers from donor to recipient cells through C×43 gap junctions.
Rechavi et al (2009) demonstrated the transfer of 22-nt Cy3-labeled dsRNAs, or possibly metabolic products of those dsRNAs, from donor B cells to recipient T cells. However, 22-nt FITC-conjugated locked nucleic acids did not transfer between cells, suggesting that structural specificity is involved in intercellular RNA transfer (Rechavi et al, 2009). Additionally, Rechavi et al (2009) did not detect the movement of the RISC component Ago2 between donor and recipient cells, suggesting that small RNAs transfer between cells independently of Ago2. Based on these studies, we hypothesize that the antisense strand of Dicer-processed siRNA may be the RNA silencing signal that transfers between cells in non-cell-autonomous RNAi; however, we recognize that there may be different RNA silencing signals depending on the mechanism of non-cell-autonomous RNAi.
There is increasing data linking RNAi to endolysosomal trafficking (reviewed in Siomi and Siomi, 2009; Gibbings and Voinnet, 2010). In the endolysosomal pathway, the endosomal sorting complex required for transport (ESCRT) is required for the invagination of the limiting endosomal membrane in MVBs to form ILVs (reviewed in Babst, 2005). ESCRT is also required for the sorting of endosomal cargo proteins into ILVs. MVBs can fuse with the lysosome for degradation or they can fuse with the plasma membrane to secrete their ILVs, which are then referred to as exosomes.
Gibbings et al (2009) demonstrated that RISC proteins GW182 and Ago2 were present with miRNAs in endosomes/MVBs from cultured monocytes, suggesting that miRNAs and RISC congregate on endosomes and/or MVBs. Furthermore, Lee et al (2009) discovered that in cultured HeLa cervical cancer cells, blocking the maturation of MVBs into lysosomes with Hermansky-Pudlak Syndrome 4 mutants stimulated RNAi. These findings, together with data showing that exosomes contain miRNAs (Valadi et al, 2007; Skog et al, 2008; Taylor and Gercel-Taylor, 2008; Luo et al, 2009; Kosaka et al, 2010; Michael et al, 2010; Ohshima et al, 2010; Pegtel et al, 2010; Zomer et al, 2010; DeIuliis et al, 2011; Levänen et al, 2011; Mittelbrunn et al, 2011), suggest that molecular pathways control the packaging of miRNA and RISC components into endosomes/MVBs and mediate intercellular transfer of RNAi (reviewed in Siomi and Siomi, 2009; Gibbings and Voinnet, 2010). However, the details of such pathways remain unclear. Although the ESCRT machinery appears to be involved in MVB formation (reviewed in Babst, 2005), evidence suggests that sphingomyelinase 2, which regulates ceramide biosynthesis, but not the ESCRT machinery, is involved in the secretion of miRNA-containing exosomes (Kosaka et al, 2010; Mittelbrunn et al, 2011).
Despite the enormous potential of RNAi for disease therapy, current in vivo RNAi delivery strategies (reviewed in Whitehead et al, 2009; Lares et al, 2010), such as those using liposomes, cationic polymers, or viral vectors, have been hindered by a variety of challenges (reviewed in Trehan et al, 2010), including immune system activation and inefficient cell targeting. Cell-based RNAi delivery, in which donor cells act as RNAi delivery vehicles, can potentially overcome these challenges (reviewed in Brink et al, 2010; Brink et al, 2011). Autologous or immunoprivileged allogeneic donor cells may avoid host immune system activation, and certain cell types inherently migrate to tumors or wounds. Furthermore, in areas where traditional RNAi delivery vehicles have limited diffusion, the spread of RNAi may be more efficient by transferring the RNA silencing signal from cell to cell.
MSCs are excellent candidates for in vivo cell-based RNAi delivery because they exhibit innate tumor- and wound-homing abilities, are considered immunoprivileged, and can be isolated in large numbers from bone marrow or adipose tissue (reviewed in Brink et al, 2010; Dwyer et al, 2010, Hu et al, 2010). Furthermore, MSCs express C×43 (Valiunas et al, 2004), allowing for the transfer of RNAi from MSCs to C×43-expressing recipient cells by GJIC. MSCs can also form TNTs (Plotnikov et al, 2010), and release miRNA-containing MVs (Collino et al, 2010) and exosomes (DeIuliis et al, 2011), illustrating their potential to participate in non-cell-autonomous RNAi by multiple mechanisms.
Emerging evidence demonstrates that non-cell-autonomous RNAi occurs in cultured mammalian cells. This phenomenon can occur by cell contact-independent mechanisms (Figure 2A-D), such as the shuttling of miRNA-containing MVs, exosomes, or apoptotic bodies between cells. Non-cell-autonomous RNAi can also occur by cell contact-dependent mechanisms, relying on TNTs, gap junctions, or ISs (Figure 2F-H). Non-cell-autonomous RNAi in mammalian cells may allow for the development of in vivo cell-based RNAi delivery, which has the potential to overcome major challenges in RNAi delivery and allow for effective RNAi therapies.
Notes STATEMENT OF COMPETING INTERESTS
We would like to acknowledge Sally Griffith-Oh at the University of Wisconsin-Madison for help in creating Figure 2. This work was supported with start-up funds from the University of Wisconsin-Madison School of Pharmacy and through a Department of Defense (DoD) National Defense Science and Engineering Graduate (NDSEG) Fellowship to HC Cohen.
|CXCL12||chemokine CXC motif ligand 12|
|ESCMVs||embryonic stem cell-derived MVs HPCs; hematopoietic progenitor cells|
|ESCRT||endosomal sorting complex required for transport|
|GFP||green fluorescent protein|
|GJIC||gap junction intercellular communication, NRK; normal rat kidney|
|hESCs||human embryonic stem cells|
|MSCs||mesenchymal stem cells|
|NRVMs||neonatal rat ventricular myocytes|
|RdRP||RNA-dependent RNA polymerase|
|RISC||RNA-induced silencing complex|
|Arroyo JD,Chevillet JR,Kroh EM,et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma.Proc Natl Acad Sci USAYear: 20111085003500821383194|
|Babst M. A protein's final ESCRT.TrafficYear: 200562915569240|
|Belting M,Wittrup A. Nanotubes, exosomes, and nucleic acid-binding peptides provide novel mechanisms of intercellular communication in eukaryotic cells: implications in health and disease.J Cell BiolYear: 20081831187119119103810|
|Bernstein E,Caudy AA,Hammond SM,Hannon GJ. Role for a bidentate ribonuclease in the initiation step of RNA interference.NatureYear: 200140936336611201747|
|Brink PR,Robinson RB,Rosen MR,Cohen IS. In vivo cellular delivery of siRNA.IDrugsYear: 20101338338720506060|
|Brink PR,Valiunas V,Gordon C,Rosen MR,Cohen IS. Can gap junctions deliver?BBA – BiomembranesYear: 2011 in press.|
|Bruzzone R,White TW,Paul DL. Connections with connexins: the molecular basis of direct intercellular signaling.Eur J BiochemYear: 19962381278665925|
|Chinnery HR,Pearlman E,McMenamin PG. Cutting edge: membrane nanotubes in vivo: a feature of MHC class II cells in the mouse cornea.J ImmunolYear: 20081805779578318424694|
|Chitwood DH,Timmermans MCP. Small RNAs are on the move.NatureYear: 201046741541920864994|
|Cocucci E,Racchetti G,Meldolesi J. Shedding microvesicles: artefacts no more.Trends Cell BiolYear: 200919435119144520|
|Collino F,Deregibus MC,Bruno S,et al. Microvesicles derived from adult human bone marrow and tissue specific mesenchymal stem cells shuttle selected pattern of miRNAs.PLoS OneYear: 20105e1180320668554|
|DeIuliis G,Di Giuseppe M,Phinney D,Sala E,Kaminski N,Ortiz LA. Differential expression of microRNA in mesenchymal stem cell derived exosomes.Am J Respir Crit Care MedYear: 2011183A3769|
|Dinger ME,Mercer TR,Mattick JS. RNAs as extracellular signaling molecules.J Mol EndocrinolYear: 20084015115918372404|
|Dunoyer P,Himber C,Voinnet O. DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal.Nat GenetYear: 2005371356136016273107|
|Dunoyer P,Schott G,Himber C,et al. Small RNA duplexes function as mobile silencing signals between plant cells.ScienceYear: 201032891291620413458|
|Duxbury MS,Ashley SW,Whang EE. RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells.Biochem Biophys Res CommunYear: 200533145946315850781|
|Dwyer RM,Khan S,Barry FP,O'Brien T,Kerin MJ. Advances in mesenchymal stem cell-mediated gene therapy for cancer.Stem Cell Res TherYear: 201012520699014|
|Fabian MR,Sonenberg N,Filipowicz W. Regulation of mRNA translation and stability by microRNAs.Annu Rev BiochemYear: 20107935137920533884|
|Feinberg EH,Hunter CP. Transport of dsRNA into cells by the transmembrane protein SID-1.ScienceYear: 20033011545154712970568|
|Fire A,Xu S,Montgomery MK,Kostas SA,Driver SE,Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.NatureYear: 19983918068119486653|
|Gerdes HH,Carvalho RN. Intercellular transfer mediated by tunneling nanotubes.Curr Opin Cell BiolYear: 20082047047518456488|
|Gibbings DJ,Ciaudo C,Erhardt M,Voinnet O. Multivesicular bodies associate with components of miRNA effector complexes and modulate miRNA activity.Nat Cell BiolYear: 2009111143114919684575|
|Gibbings D,Voinnet O. Control of RNA silencing and localization by endolysosomes.Trends Cell BiolYear: 20102049150120630759|
|Gurke S,Barroso JF,Gerdes HH. The art of cellular communication: tunneling nanotubes bridge the divide.Histochem Cell BiolYear: 200812953955018386044|
|Hammond SM,Bernstein E,Beach D,Hannon GJ. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells.NatureYear: 200040429329610749213|
|Hand GM,Muller DJ,Nicholson BJ,Engel A,Sosinsky GE. Isolation and characterization of gap junctions from tissue culture cells.J Mol BiolYear: 200231558760011812132|
|He L,Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation.Nature Rev GenetYear: 2004552253115211354|
|Himber C,Dunoyer P,Moissiard G,Ritzenthaler C,Voinnet O. Transitivity-dependent and -independent cell-to-cell movement of RNA silencing.EMBO JYear: 2003224523453312941703|
|Hu Y,Fu Y,Tabata Y,Gao J. Mesenchymal stem cells: a promising targeted-delivery vehicle in cancer gene therapy.J Control ReleaseYear: 201014715416220493219|
|Hunter MP,Ismail N,Zhang X,et al. Detection of microRNA expression in human peripheral blood microvesicles.PLoS OneYear: 20083e369419002258|
|Hutvágner G,Zamore PD. A microRNA in a multiple-turnover RNAi enzyme complex.ScienceYear: 20022972056206012154197|
|Jose AM,Hunter CP. Transport of sequence-specific RNA interference information between cells.Annu Rev GenetYear: 20074130533017645412|
|Kalantidis K,Schumacher HT,Alexiadis T,Helm JM. RNA silencing movement in plants.Biol CellYear: 2008100132618072941|
|Kizana E,Chang CY,Cingolani E,et al. Gene transfer of connexin43 mutants attenuates coupling in cardiomyocytes.Circ ResYear: 20071001597160417495226|
|Kizana E,Cingolani E,Marban E. Non-cell-autonomous effects of vector-expressed regulatory RNAs in mammalian heart cells.Gene TherYear: 2009161163116819516277|
|Kosaka N,Iguchi H,Yoshioka Y,Takeshita F,Matsuki Y,Ochiya T. Secretory mechanisms and intercellular transfer of microRNAs in living cells.J Biol ChemYear: 2010285174421745220353945|
|Lares MR,Rossi JJ,Ouellet DL. RNAi and small interfering RNAs in human disease therapeutic applications.Trends BiotechnolYear: 20102857057920833440|
|Lee YS,Pressman S,Andress AP,et al. Silencing by small RNAs is linked to endosomal trafficking.Nat Cell BiolYear: 2009111150115619684574|
|Levänen B,Torregrosa-Paredes P,Barbeau R,et al. Differences in exosomal microRNAs in bronchoalveolar lavage fluid from asthmatics and healthy individuals.Am J Respir Crit Care MedYear: 2011183A1006|
|Lim PK,Bliss SA,Patel SA,et al. Gap junction-mediated import of microRNA from bone marrow stromal cells can elicit cell cycle quiescence in breast cancer cells.Cancer ResYear: 2011711550156021343399|
|Liu J,Carmell MA,Rivas FV,et al. Argonaute2 is the catalytic engine of mammalian RNAi.ScienceYear: 20043051437144115284456|
|Lotvall J,Valadi H. Cell to cell signalling via exosomes through esRNA.Cell Adh MigrYear: 2007115615819262134|
|Lu JJ,Langer R,Chen J. A novel mechanism is involved in cationic lipid-mediated functional siRNA delivery.Mol PharmYear: 2009676377119292453|
|Luo SS,Ishibashi O,Ishikawa G,et al. Human villous trophoblasts express and secrete placenta-specific microRNAs into maternal circulation via exosomes.Biol ReprodYear: 20098171772919494253|
|Maida Y,Yasukawa M,Furuuchi M,et al. An RNA-dependent RNA polymerase formed by TERT and the RMRP RNA.NatureYear: 200946123023519701182|
|Manche L,Green SR,Schmedt C,Mathews MB. Interactions between double-stranded RNA regulators and the protein kinase DAI.Mol Cell BiolYear: 199212523852481357546|
|Martinez J,Patkaniowska A,Urlaub H,Lührmann R,Tuschl T. Single-stranded antisense siRNAs guide target RNA cleavage in RNAi.CellYear: 200211056357412230974|
|Matranga C,Tomari Y,Shin C,et al. Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.CellYear: 200512360762016271386|
|Michael A,Bajracharya SD,Yuen PST,et al. Exosomes from human saliva as a source of microRNA biomarkers.Oral DisYear: 201016343819627513|
|Mincheva-Nilsson L,Baranov V. The role of placental exosomes in reproduction.Am J Reprod ImmunolYear: 20106352053320331583|
|Minks MA,West DK,Benvin S,Baglioni C. Structural requirements of double-stranded RNA for the activation of 2′, 5′-oligo (A) polymerase and protein kinase of interferon-treated HeLa cells.J Biol ChemYear: 19792541018010183489592|
|Mitchell GA,Olson SD,Pollock KM,et al. Mesenchymal stem cells as a delivery vehicle for intercellular delivery of RNAi to treat Huntington's disease.AAN, IN10-1.010Year: 2011|
|Mittelbrunn M,Gutiérrez-Vázquez C,Villarroya-Beltri C,et al. Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.Nat CommunYear: 2011228221505438|
|Mlotshwa S,Voinnet O,Mette MF,et al. RNA silencing and the mobile silencing signal.Plant CellYear: 200214SupplS289S30112045284|
|Nishikura K. A short primer on RNAi: RNA-directed RNA polymerase acts as a key catalyst.CellYear: 200110741541811719182|
|Ohshima K,Inoue K,Fujiwara A,et al. Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line.PLoS OneYear: 20105e1324720949044|
|Olson SD,Pollock K,McNerny G,Chuang F,Huser T,Nolta JA. Visualization of siRNA complexed to RISC machinery: demonstrating intercellular siRNA transfer by directly imaging activity.AAN, IN4-1.014Year: 2011|
|Önfelt B,Nedvetzki S,Benninger RK,et al. Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria.J ImmunolYear: 20061778476848317142745|
|Pegtel DM,Cosmopoulos K,Thorley-Lawson DA,et al. Functional delivery of viral miRNAs via exosomes.Proc Natl Acad Sci USAYear: 20101076328633320304794|
|Plotnikov EY,Khryapenkova TG,Galkina SI,Sukhikh GT,Zorov DB. Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture.Exp Cell ResYear: 20103162447245520599955|
|Rand TA,Ginalski K,Grishin NV,Wang X. Biochemical identification of Argonaute 2 as the sole protein required for RNA-induced silencing complex activity.Proc Natl Acad Sci U S AYear: 2004101143851438915452342|
|Ratajczak J,Miekus K,Kucia M,et al. Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery.LeukemiaYear: 2006a2084785616453000|
|Ratajczak J,Wysoczynski M,Hayek F,Janowska-Wieczorek A,Ratajczak MZ. Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication.LeukemiaYear: 2006b201487149516791265|
|Rechavi O,Erlich Y,Amram H,et al. Cell contact-dependent acquisition of cellular and viral nonautonomously encoded small RNAs.Genes DevYear: 2009231971197919684116|
|Rodríguez-Fernández JL,Riol-Blanco L,Delgado-Martín C. What is an immunological synapse?Microb InfectYear: 201012438445|
|Rustom A. Hen or Egg? Some thoughts on tunneling nanotubes.Ann N Y Acad SciYear: 2009117812913619845633|
|Rustom A,Saffrich R,Markovic I,Walther P,Gerdes HH. Nanotubular highways for intercellular organelle transport.ScienceYear: 20043031007101014963329|
|Sijen T,Fleenor J,Simmer F,et al. On the role of RNA amplification in dsRNA-triggered gene silencing.CellYear: 200110746547611719187|
|Simons M,Raposo G. Exosomes – vesicular carriers for intercellular communication.Curr Opin Cell BiolYear: 20092157558119442504|
|Siomi H,Siomi MC. RISC hitches onto endosome trafficking.Nat Cell BiolYear: 2009111049105119724258|
|Skog J,Würdinger T,Van Rijn S,et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers.Nat Cell BiolYear: 2008101470147619011622|
|Smalheiser N. Exosomal transfer of proteins and RNAs at synapses in the nervous system.Biol DirectYear: 200723518053135|
|Taylor DD,Gercel-Taylor C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer.Gynecol OncolYear: 2008110132118589210|
|Théry C,Zitvogel L,Amigorena S. Exosomes: composition, biogenesis and function.Nat Rev ImmunolYear: 2002256957912154376|
|Timmons L,Fire A. Specific interference by ingested dsRNA.NatureYear: 19983958549804418|
|Trehan S,Sharma G,Misra A. siRNA: sojourn from discovery to delivery challenges and clinics.Syst Rev PharmYear: 20101116|
|Tsang SY,Moore JC,Huizen RV,Chan CWY,Li RA. Ectopic expression of systemic RNA interference defective protein in embryonic stem cells.Biochem Biophys Res CommunYear: 200735748048617434453|
|Valadi H,Ekstrom K,Bossios A,Sjostrand M,Lee JJ,Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.Nat Cell BiolYear: 2007965465917486113|
|Valiunas V,Doronin S,Valiuniene L,et al. Human mesenchymal stem cells make cardiac connexins and form functional gap junctions.J PhysiolYear: 200455561762614766937|
|Valiunas V,Polosina YY,Miller H,et al. Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions.J PhysiolYear: 200556845946816037090|
|Voinnet O,Vain P,Angell S,Baulcombe DC. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA.CellYear: 1998951771879790525|
|Voinnet O. Non-cell autonomous RNA silencing.FEBS LettYear: 20055795858587116242131|
|Winston WM,Molodowitch C,Hunter CP. Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.ScienceYear: 20022952456245911834782|
|Whitehead KA,Langer R,Anderson DG. Knocking down barriers: advances in siRNA delivery.Nat Rev Drug DiscovYear: 2009812913819180106|
|Wolvetang EJ,Pera MF,Zuckerman KS. Gap junction mediated transport of shRNA between human embryonic stem cells.Biochem Biophys Res CommunYear: 200736361061517900528|
|Yuan A,Farber EL,Rapoport AL,et al. Transfer of microRNAs by embryonic stem cell microvesicles.PLoS OneYear: 20094e472219266099|
|Zamore PD,Tuschl T,Sharp PA,Bartel DP. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.CellYear: 2000101253310778853|
|Zernecke A,Bidzhekov K,Noels H,et al. Delivery of microRNA-126 by apoptotic bodies induces CXCL12-dependent vascular protection.Sci SignalYear: 20092ra8119996457|
|Zomer A,Vendrig T,Hopmans ES,van Eijndhoven M,Middeldorp JM,Pegtel DM. Exosomes: fit to deliver small RNA.Commun Integr BiolYear: 2010344745021057637|
[Figure ID: F1]
The RNAi pathway. Dicer cleaves long dsRNA in the form of pre-shRNA, pre-miRNA, or Dicer-substrate siRNA into ∼21–25-nt dsRNA duplexes (Hammond et al, 2000; Zamore et al, 2000), referred to as shRNAs, miRNAs, or siRNAs, respectively, to initiate RNAi. Most shRNAs and siRNAs have exact complementarity with their target mRNAs and result in mRNA cleavage by Ago2. Most miRNAs bind to the 3' untranslated region of their target mRNAs with imperfect complementarity, resulting in translational repression (reviewed in He and Hannon, 2004) or deadenylation and target mRNA degradation (reviewed in Fabian et al, 2010). Alternatively, ∼21–25nt siRNAs can be directly transfected into the cytoplasm of cells to initiate RNAi. Note that the term siRNA can also be used more generally to describe any of the forms of ∼21–25nt dsRNA duplexes that initiate RNAi.
[Figure ID: F2]
Possible mechanisms of non-cell-autonomous RNAi in cultured mammalian cells. Non-cell-autonomous RNAi can occur by cell contact-independent mechanisms, such as the shuttling of miRNA-containing MVs (A), exosomes (B), or apoptotic bodies (C) between cells. Additionally, miRNA-Ago2 complexes may induce RNAi in recipient cells (D). siRNAs and miRNAs may also utilize SIDT-1, when present at high expression levels, for entry into cells and to generate RNAi (E). Non-cell-autonomous RNAi can also occur by cell contact-dependent mechanisms, relying on TNTs (F), gap junctions (G), or ISs (H).
[Figure ID: F3]
The predicted transfer of RNAi when the ratio of siRNA-expressing donor cells to recipient cells in co-culture is increased. Valiunas et al (2005) observed a greater percentage of target gene knockdown in recipient cells when the ratio of donor to recipient cells increased from 1:1 to 2:1. Similarly, Wolvetang et al (2007) observed a progressive increase in target gene knockdown in recipient cells when the ratio of donor to recipient cells increased from 1:1 to 2:1 to 3:1. By increasing the ratio of donor to recipient cells, we expect to eventually reach a maximum percentage of target gene knockdown in recipient cells. In cell contact-dependent transfer of RNAi, such as by GJIC, limited points of contact between the donor and recipient cells may reduce the maximum target gene knockdown in recipient cells.
Keywords: RNAi, siRNA, miRNA, non-cell-autonomous RNAi, systemic RNAi, RNAi delivery, cell-based delivery.
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