| Non-aqueous capillary electrophoresis using non-dissociating solvents. Application to the separation of highly hydrophobic oligomers. | |
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MedLine Citation:
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PMID: 11358254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The application of non-aqueous capillary electrophoresis for the separation of very hydrophobic oligomers has been studied. N-Phenylaniline oligomers having degrees of polymerisation (n) of 2, 4, 6, and 8 were taken as model compounds. Capillary electrophoresis could be performed using a mixture of non-aqueous solvents with a high percentage of solvents with a low dielectric constant. These solvents, such as tetrahydrofuran (THF), chloroform or dichloromethane, are needed to solubilise the hydrophobic solutes in the electrolyte. The composition of the solvent mixture and the nature of the acid added to the electrolyte, which is needed to obtain electrophoretic motion of the N-phenylaniline oligomers, are discussed in detail. Next, other parameters such as ionic strength, injection time, electric field, and temperature were investigated too and their influence on the separation is discussed as well. The existence of a reversed (anodic) electroosmotic flow in a fused-silica capillary containing a THF-methanol mixture under acidic conditions is reported. |
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Authors:
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H Cottet; M P Struijk; J L Van Dongen; H A Claessens; C A Cramers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatography. A Volume: 915 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-05-18 Completed Date: 2001-09-13 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 241-51 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Eindhoven University of Technology, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chloroform
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chemistry Electrophoresis, Capillary / methods* Furans / chemistry Methylene Chloride / chemistry Osmolar Concentration Polymers / chemistry, isolation & purification* Solvents / chemistry* Temperature Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Furans; 0/Polymers; 0/Solvents; 109-99-9/tetrahydrofuran; 67-66-3/Chloroform; 75-09-2/Methylene Chloride; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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