Document Detail


Non-anti-mitotic concentrations of taxol reduce breast cancer cell invasiveness.
MedLine Citation:
PMID:  19111674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Taxol is widely used in breast cancer chemotherapy. Its effects are primarily attributed to its anti-mitotic activity. Microtubule perturbators also exert antimetastatic activities which cannot be explained solely by the inhibition of proliferation. Voltage-dependent sodium channels (Na(V)) are abnormally expressed in the highly metastatic breast cancer cell line MDA-MB-231 and not in MDA-MB-468 cell line. Inhibiting Na(V) activity with tetrodotoxin is responsible for an approximately 0.4-fold reduction of MDA-MB-231 cell invasiveness. In this study, we focused on the effect of a single, 2-h application of 10 nM taxol on the two cell lines MDA-MB-231 and MDA-MB-468. At this concentration, taxol had no effect on proliferation after 7 days and on migration in any cell line. However it led to a 40% reduction of transwell invasion of MDA-MB-231 cells. There was no additive effect when taxol and tetrodotoxin were simultaneously applied. Na(V) activity, as assessed by patch-clamp, indicates that it was changed by taxol pre-treatment. We conclude that taxol can exert anti-tumoral activities, in cells expressing Na(V), at low doses that have no effect on cell proliferation. This effect might be due to a modulation of signalling pathways involving sodium channels.
Authors:
Truong-An Tran; Ludovic Gillet; Sébastien Roger; Pierre Besson; Edward White; Jean-Yves Le Guennec
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-26
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  379     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-02-13     Revised Date:  2011-07-22    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  304-8     Citation Subset:  IM    
Affiliation:
Inserm, U921, 37000 Tours, France.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology*
Breast Neoplasms / pathology*
Cell Line, Tumor
Cell Proliferation / drug effects
Humans
Mitosis / drug effects
Neoplasm Invasiveness
Paclitaxel / pharmacology*
Signal Transduction / drug effects
Sodium Channels / drug effects
Tubulin Modulators / pharmacology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Sodium Channels; 0/Tubulin Modulators; 0/sodium channel protein type 5 subunit alpha; 33069-62-4/Paclitaxel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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