Document Detail

Non-aggregated protamine-coated poly(lactide-co-glycolide) nanoparticles of cisplatin crossed blood-brain barrier, enhanced drug delivery and improved therapeutic index in glioblastoma cells: in vitro studies.
MedLine Citation:
PMID:  24963955     Owner:  NLM     Status:  Publisher    
Abstract Background and objectives: Non-aggregated protamine impregnated poly(lactide-co-glycolide) nanoparticles of cisplatin (Pt-PLGA NPs) were synthesized to augment brain delivery. Methods and results: The mean particle size of Pt-PLGA NPs and PLGA NPs were observed to be 173.2 ± 7.9 nm and 140 ± 10.2 nm, respectively. The Pt-PLGA NPs significantly (p < 0.05, one-way analysis of variance; ANOVA) delivered higher amount (172.41 ± 15.04 μg) of cisplatin in comparison to 110.48 ± 4.71 μg by PLGA NPs and 20.83 ± 1.65 μg by cisplatin solution across in vitro bovine brain microvessel endothelial cells. Cisplatin bearing Pt-PLGA NPs was found to be highly cytotoxic to U87 glioblastoma cells with an IC50 of 2.1 μM as compared (one-way ANOVA, p < 0.05) to PLGA NPs (3.9 μM) and cisplatin alone (13.33 μM). Impregnation with Pt enhanced the uptake of PLGA NPs in U87 glioblastoma cells as compared to PLGA NPs by following endocytosis mechanism. Conclusion: Cisplatin-loaded Pt-PLGA NPs compel preclinical tumour regression study to further improve its utility against glioblastoma.
Neel Kamal Dhami; Ravi Shankar Pandey; Upendra Kumar Jain; Ramesh Chandra; Jitender Madan
Related Documents :
24152735 - The rac-gap bcr is a novel regulator of the par complex that controls cell polarity.
23093325 - Fabrication and use of microenvironment microarrays (mearrays).
24460265 - Inhibition of nemo-like kinase increases taxol sensitivity in laryngeal cancer.
23420395 - Anticancer effects of 3,3'-diindolylmethane are associated with g1 arrest and mitochond...
7722335 - Murine and human antibodies to native dna that cross-react with the a and d snrnp polyp...
15557385 - In vivo tracking of stem cells for clinical trials in cardiovascular disease.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-25
Journal Detail:
Title:  Journal of microencapsulation     Volume:  -     ISSN:  1464-5246     ISO Abbreviation:  J Microencapsul     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500513     Medline TA:  J Microencapsul     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-9     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Perfluorodecalin-soluble fluorescent dyes for the monitoring of circulating nanocapsules with intrav...
Next Document:  Ethosomes-based topical delivery system of antihistaminic drug for treatment of skin allergies.