Document Detail


Non-additive phenotypic and transcriptomic inheritance in a citrus allotetraploid somatic hybrid between C. reticulata and C. limon: the case of pulp carotenoid biosynthesis pathway.
MedLine Citation:
PMID:  19727737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Allopolyploidy is known to induce novel patterns of gene expression and often gives rise to new phenotypes. Here we report on the first attempt to relate phenotypic inheritance in an allotetraploid somatic hybrid with gene expression. Carotenoid compounds in the fruit pulp of the two parental species and the hybrid were evaluated quantitatively by HPLC. Only very low levels of beta-carotene and beta-cryptoxanthin were observed in Citrus limon, while beta-cryptoxanthin was a major component of C. reticulata, which also displayed high levels of phytoene, phytofluene, beta-carotene, lutein, zeaxantin and violaxanthin. Total carotenoid content in mandarin juice sacs was 60 times greater than that in lemon. The allotetraploid hybrid produced all the same compounds as mandarin but at very low levels. Transgressive concentration of abscisic acid (ABA) was observed in the somatic hybrid. Real-time RT-PCR of total RNA from juice sacs was used to study expression of seven genes (CitDxs, CitPsy, CitPds, CitZds, CitLcy-b, CitChx-b, and CitZep) of the carotenoid biosynthetic pathway and two genes (CitNced1 and CitNced2) involved in abscisic acid synthesis from carotenoid. Gene expression was significantly higher for mandarin than lemon for seven of the nine genes analyzed. Lemon under expression was partially dominant in the somatic hybrid for three upstream steps of the biosynthetic pathway, particularly for CitDxs. Transgressive over expression was observed for the two CitNced genes. A limitation of the upstream steps of the pathway and a downstream higher consumption of carotenoids may explain the phenotype of the somatic hybrid.
Authors:
Jean Baptiste Bassene; Yann Froelicher; Claudie Dhuique-Mayer; Waffa Mouhaya; Rosa Mar Ferrer; Gema Ancillo; Raphael Morillon; Luis Navarro; Patrick Ollitrault
Related Documents :
8076597 - The aspergillus nidulans crea protein mediates glucose repression of the ethanol regulo...
20618907 - The cell wall polysaccharide metabolism of the brown alga ectocarpus siliculosus. insig...
18282727 - A defect of ligd (human lig4 homolog) for nonhomologous end joining significantly impro...
19309517 - Characterization of oxylipins and dioxygenase genes in the asexual fungus aspergillus n...
24356447 - Sirt1 gene expression upon genotoxic damage is regulated by ape1 through ncare-promoter...
16405867 - Association study of serotonin 2a receptor (5-ht2a) gene with schizophrenia and suicida...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-29
Journal Detail:
Title:  Plant cell reports     Volume:  28     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1689-97     Citation Subset:  IM    
Affiliation:
UPR Am?lioration g?n?tique des esp?ces ? multiplication v?g?tative, Centre de Coop?ration Internationale en Recherche Agronomique pour le D?veloppement, San Giuliano, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Ascorbic Acid / biosynthesis
Carotenoids / biosynthesis*
Chimera
Citrus / anatomy & histology,  genetics*,  metabolism
Gene Expression Regulation, Plant
Phenotype
Phylogeny
Plant Proteins / genetics*,  metabolism
Polyploidy*
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Plant Proteins; 36-88-4/Carotenoids; 50-81-7/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Psychometric properties evaluation of the childhood health assessment questionnaire (CHAQ) in Morocc...
Next Document:  Introduction of transformed chloroplasts from tobacco into petunia by asymmetric cell fusion.