| Non-lytic, actin-based exit of intracellular parasites from C. elegans intestinal cells. | |
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MedLine Citation:
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PMID: 21949650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intestine is a common site for invasion by intracellular pathogens, but little is known about how pathogens restructure and exit intestinal cells in vivo. The natural microsporidian parasite N. parisii invades intestinal cells of the nematode C. elegans, progresses through its life cycle, and then exits cells in a transmissible spore form. Here we show that N. parisii causes rearrangements of host actin inside intestinal cells as part of a novel parasite exit strategy. First, we show that N. parisii infection causes ectopic localization of the normally apical-restricted actin to the basolateral side of intestinal cells, where it often forms network-like structures. Soon after this actin relocalization, we find that gaps appear in the terminal web, a conserved cytoskeletal structure that could present a barrier to exit. Reducing actin expression creates terminal web gaps in the absence of infection, suggesting that infection-induced actin relocalization triggers gap formation. We show that terminal web gaps form at a distinct stage of infection, precisely timed to precede spore exit, and that all contagious animals exhibit gaps. Interestingly, we find that while perturbations in actin can create these gaps, actin is not required for infection progression or spore formation, but actin is required for spore exit. Finally, we show that despite large numbers of spores exiting intestinal cells, this exit does not cause cell lysis. These results provide insight into parasite manipulation of the host cytoskeleton and non-lytic escape from intestinal cells in vivo. |
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Authors:
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Kathleen A Estes; Suzannah C Szumowski; Emily R Troemel |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-15 |
Journal Detail:
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Title: PLoS pathogens Volume: 7 ISSN: 1553-7374 ISO Abbreviation: PLoS Pathog. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-09-28 Completed Date: 2012-02-29 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 101238921 Medline TA: PLoS Pathog Country: United States |
Other Details:
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Languages: eng Pagination: e1002227 Citation Subset: IM |
Affiliation:
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Division of Biological Sciences, University of California, San Diego, La Jolla, California, United States of America. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actin Cytoskeleton
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metabolism*,
microbiology Animals Caenorhabditis elegans / cytology, microbiology* Host-Pathogen Interactions Intestines / cytology, parasitology Microfilament Proteins / metabolism Microsporidia / metabolism, pathogenicity* RNA Interference RNA, Small Interfering Spores, Fungal / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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5T32AI060536/AI/NIAID NIH HHS; AI087528/AI/NIAID NIH HHS; R01 AI087528-03/AI/NIAID NIH HHS; T32GM008666/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Microfilament Proteins; 0/RNA, Small Interfering |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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