| Noise induced changes in the expression of p38/MAPK signaling proteins in the sensory epithelium of the inner ear. | |
| | |
MedLine Citation:
|
PMID: 21871588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Noise exposure is a major cause of hearing loss. Classical methods of studying protein involvement have provided a basis for understanding signaling pathways that mediate hearing loss and damage repair but do not lend themselves to studying large networks of proteins that are likely to increase or decrease during noise trauma. To address this issue, antibody microarrays were used to quantify the very early changes in protein expression in three distinct regions of the chinchilla cochlea 2h after exposure to a 0.5-8 kHz band of noise for 2h at 112 dB SPL. The noise exposure caused significant functional impairment 2h post-exposure which only partially recovered. Distortion product otoacoustic emissions were abolished 2h after the exposure, but at 4 weeks post-exposure, otoacoustic emissions were present, but still greatly depressed. Cochleograms obtained 4 weeks post-exposure demonstrated significant loss of outer hair cells in the basal 60% of the cochlea corresponding to frequencies in the noise spectrum. A comparative analysis of the very early (2h post-exposure) noise-induced proteomic changes indicated that the sensory epithelium, lateral wall and modiolus differ in their biological response to noise. Bioinformatic analysis of the cochlear protein profile using "The Database for Annotation, Visualization and Integrated Discovery 2008" (DAVID - http://david.abcc. ncifcrf.gov) revealed the initiation of the cell death process in sensory epithelium and modiolus. An increase in Fas and phosphorylation of FAK and p38/MAPK in the sensory epithelium suggest that noise-induced stress signals at the cell membrane are transmitted to the nucleus by Fas and focal adhesion signaling through the p38/MAPK signaling pathway. Up-regulation of downstream nuclear proteins E2F3 and WSTF in immunoblots and microarrays along with their immunolocalization in the outer hair cells supported the pivotal role of p38/MAPK signaling in the mechanism underlying noise-induced hearing loss. |
| | |
Authors:
|
Samson Jamesdaniel; Bohua Hu; Mohammad Habiby Kermany; Haiyan Jiang; Dalian Ding; Donald Coling; Richard Salvi |
Related Documents
:
|
7146908 - Fish vision and the detection of planktonic prey. 17522478 - Cone photoreceptor diversity in the retinas of fruit bats (megachiroptera). 2085468 - Iso-orientation areas in the foveal cone mosaic. 1771798 - The invariance of unique white; a possible implication for normalizing cone action spec... 8317758 - External loads on the limbs of jumping horses at take-off and landing. 20622748 - Influence of number of operated levels and postoperative time on active range of motion... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-08-16 |
Journal Detail:
|
Title: Journal of proteomics Volume: 75 ISSN: 1876-7737 ISO Abbreviation: J Proteomics Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-11-28 Completed Date: 2012-03-29 Revised Date: 2012-04-26 |
Medline Journal Info:
|
Nlm Unique ID: 101475056 Medline TA: J Proteomics Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 410-24 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
|
Center for Hearing and Deafness, University at Buffalo, the State University of New York, Buffalo, NY 14214, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD95 / biosynthesis Chinchilla Cochlea / injuries, physiopathology* Focal Adhesion Protein-Tyrosine Kinases / metabolism Hair Cells, Auditory, Outer / metabolism*, pathology Hearing Loss, Noise-Induced / physiopathology* Noise Otoacoustic Emissions, Spontaneous / physiology Proteomics Signal Transduction / physiology* p38 Mitogen-Activated Protein Kinases / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
|
1R01DC009219/DC/NIDCD NIH HHS; R01 DC009091-04/DC/NIDCD NIH HHS; R01 DC009219-04/DC/NIDCD NIH HHS; R01DC009091/DC/NIDCD NIH HHS; R03 DC010225/DC/NIDCD NIH HHS; R03 DC010225-02/DC/NIDCD NIH HHS; R03DC010225/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD95; EC 2.7.10.2/Focal Adhesion Protein-Tyrosine Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Precipitation by lanthanum ions: A straightforward approach to isolating phosphoproteins.
Next Document: A randomized, double-blind, placebo-controlled, single-dose study to evaluate the safety, tolerabili...