Document Detail


Noise Adjusted Principal Component reconstruction to optimize infrared microspectroscopy of individual live cells.
MedLine Citation:
PMID:  22377958     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have optimized an imaging methodology capable of monitoring individual live HeLa cells using non-synchrotron FTIR in an aqueous environment. This methodology, in combination with MATLAB based pre-processing techniques, allows fast and efficient collection of data with high signal-to-noise ratio in comparison with previous methods using point mode data collection, which required manual operation and more collection time. Also, presented are early results that illustrate interpretable spectral differences from live cells treated with chemotherapeutic drugs, demonstrating the potential of this methodology to develop more desirable modes of treatment for patients in their diagnoses and treatments for disease.
Authors:
Ellen J Swain Marcsisin; Christina M Uttero; Antonella I Mazur; Miloš Miljković; Benjamin Bird; Max Diem
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-1
Journal Detail:
Title:  The Analyst     Volume:  -     ISSN:  1364-5528     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA. m.diem@neu.edu.
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