Document Detail


Nodulin 22, a novel small heat-shock protein of the endoplasmic reticulum, is linked to the unfolded protein response in common bean.
MedLine Citation:
PMID:  24073881     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of plant small heat shock proteins (sHsp) in multiple cellular processes has been evidenced by their unusual abundance and diversity; however, little is known about their biological role. Here, we characterized the in vitro chaperone activity and subcellular localization of nodulin 22 of Phaseolus vulgaris (PvNod22; common bean) and explored its cellular function through a virus-induced gene silencing-based reverse genetics approach. We established that PvNod22 facilitated the refolding of a model substrate in vitro, suggesting that it acts as a molecular chaperone in the cell. Through microscopy analyses of PvNod22, we determined its localization in the endoplasmic reticulum (ER). Furthermore, we found that silencing of PvNod22 resulted in necrotic lesions in the aerial organs of P. vulgaris plants cultivated under optimal conditions and that downregulation of PvNod22 activated the ER-unfolded protein response (UPR) and cell death. We also established that PvNod22 expression in wild-type bean plants was modulated by abiotic stress but not by chemicals that trigger the UPR, indicating PvNod22 is not under UPR control. Our results suggest that the ability of PvNod22 to suppress protein aggregation contributes to the maintenance of ER homeostasis, thus preventing the induction of cell death via UPR in response to oxidative stress during plant-microbe interactions.
Authors:
Jonathan Rodriguez-López; Cynthia Martínez-Centeno; Annamalai Padmanaban; Gabriel Guillén; Juan Elías Olivares; Giovanni Stefano; Fernando Lledías; Fernando Ramos; Said A Ghabrial; Federica Brandizzi; Mario Rocha-Sosa; Claudia Díaz-Camino; Federico Sanchez
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  27     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2013-12-04     Completed Date:  2014-04-03     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18-29     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Death
Down-Regulation
Endoplasmic Reticulum / metabolism
Flowers / cytology,  genetics,  metabolism
Gene Expression Regulation, Plant*
Gene Silencing
Genes, Reporter
Heat-Shock Proteins, Small / genetics,  metabolism
Homeostasis
Membrane Proteins / genetics,  metabolism*
Phaseolus / cytology,  genetics*,  metabolism
Phenotype
Phylogeny
Plant Leaves / cytology,  genetics,  metabolism
Plant Proteins / genetics,  metabolism*
Plant Roots / cytology,  genetics,  metabolism
Plants, Genetically Modified
Recombinant Proteins
Seedling / cytology,  genetics,  metabolism
Signal Transduction
Stress, Physiological
Tobacco / cytology,  genetics,  metabolism
Unfolded Protein Response*
Grant Support
ID/Acronym/Agency:
R01 GM101038/GM/NIGMS NIH HHS; R01 GM101038-01/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Heat-Shock Proteins, Small; 0/Membrane Proteins; 0/Plant Proteins; 0/Recombinant Proteins; 0/nodulin
Comments/Corrections

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