Document Detail


Nocturnin in the Demosponge Suberites domuncula: a Potential Circadian Clock Protein Controlling Glycogenin Synthesis in Sponges.
MedLine Citation:
PMID:  22928820     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sponges are filter feeders that consume large amount of energy to allow a controlled filtration of water through their aquiferous canal systems. It is shown that primmorphs, three-dimensional cell aggregates prepared from the demosponge Suberites domuncula and cultured in vitro, change their morphology depending on the light supply. Upon exposure to light, primmorphs show a faster and stronger increase in DNA, protein and glycogen content compared to primmorphs that remain in the dark. The sponge genome contains nocturnin, a light/dark controlled clock gene whose deduced protein shares high sequence similarity to the related molecule of higher metazoans. The sponge nocturnin protein was found showing a poly(A) specific 3' exoribonuclease activity. In addition, the glycogenin cDNA was identified for subsequent expression studies. Antibodies against nocturnin were raised and used in parallel with the cDNA to determine the regional expression of nocturnin in intact sponge specimens; the highest expression of nocturnin was seen in the epithelial layer around the aquiferous canals. Quantitative polymerase chain reaction analyses revealed that primmorphs after transfer from light to dark show a 10-fold increased expression of nocturnin gene. In contrast, the expression level of glycogenin decreases in the dark by 3- to 4-fold. Exposure of primmorphs to light causes a decrease of nocturnin transcripts and an opposed increase in glycogenin transcripts. It is concluded that sponges are provided with the molecular circadian clock protein nocturnin that is highly expressed in the dark and controls in the dark the stability of a key metabolic enzyme, glycogenin.
Authors:
Werner E G Müller; Xiaohong Wang; Vlad A Grebenjuk; Michael Korzhev; Matthias Wiens; Ute Schlossmacher; Heinz C Schröder
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-28
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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