| Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization. | |
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MedLine Citation:
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PMID: 16432713 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been predicted that nocodazole-inhibited cells are not synchronized because nocodazole-arrested cells with a G2-phase amount of DNA would not have a narrow cell-size range reflecting the cell size of some specific, presumably G2-phase, cell-cycle age. Size measurements of nocodazole-inhibited cells now fully confirm this prediction. Further, release from nocodazole inhibition does not produce cells that move through the cell cycle mimicking the passage of normal unperturbed cells through the cell cycle. Nocodazole, an archetypal whole-culture synchronization method, can inhibit growth to produce cells with a G2-phase amount of DNA, but such cells are not synchronized. Cells produced by a selective (i.e., non-whole-culture) method not only have a specific DNA content, but also have a narrow size distribution. The current view of cell-cycle control that is based on methods that are not suitable for cell-cycle analysis must therefore be reconsidered when results are based on whole-culture synchronization. |
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Authors:
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Stephen Cooper; Geetha Iyer; Michael Tarquini; Patrick Bissett |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-01-24 |
Journal Detail:
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Title: Cell and tissue research Volume: 324 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2006 May |
Date Detail:
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Created Date: 2006-04-12 Completed Date: 2007-03-28 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 237-42 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA. cooper@umich.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques / methods Cell Cycle / drug effects* Cell Line, Tumor Cell Size / drug effects DNA, Neoplasm / metabolism G2 Phase / drug effects Leukemia L1210 / metabolism, pathology Mice Nocodazole / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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5 P30 CA46592/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 31430-18-9/Nocodazole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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