Document Detail

Nocardia asteroides culture filtrates cause dopamine depletion and cytotoxicity in PC12 cells.
MedLine Citation:
PMID:  12938858     Owner:  NLM     Status:  MEDLINE    
Experimental infection of BALB/c mice with the gram-positive bacterium Nocardia asteroides produces marked loss of nigrostriatal dopamine neurons, resulting in striatal dopamine depletion. To investigate the mechanism(s) responsible for this neuronal loss, we evaluated the influence of N. asteroides cell-free culture filtrates on rat pheochromocytoma PC12 cells, an in vitro model for dopamine neurons. Changes in cell viability and cell numbers were minimal after 24 h, but increased with longer incubation. In contrast, dopamine depletion occurred after 30 min incubation, and was greater with GUH-2 filtrate than with filtrate from the less virulent strain 10905. Incubation with the culture filtrate decreased viability in neuroblastoma and glioma cell lines, indicating that cytotoxic effects were not limited to dopaminergic cells. These findings suggest that the loss of nigrostriatal dopamine neurons and concomitant striatal dopamine depletion in Nocardia-infected mice may be due, at least in part, to the neurotoxicity of nocardial secretory products.
Dianne M Camp; David A Loeffler; Bnan A Razoky; Stanley Tam; Blaine L Beaman; Peter A LeWitt
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-26     Completed Date:  2003-09-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1359-67     Citation Subset:  IM    
Division of Neurology, William Beaumont Hospital Research Institute, Royal Oak, Michigan 48073, USA.
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MeSH Terms
Biological Factors / metabolism,  toxicity*
Catecholamines / metabolism
Cell Survival / drug effects
Culture Media
Dopamine / metabolism*
Neuroglia / cytology,  drug effects
Neurons / cytology,  drug effects*,  metabolism
Neurotoxins / metabolism,  toxicity*
Nocardia asteroides / isolation & purification,  metabolism*
PC12 Cells
Time Factors
Grant Support
Reg. No./Substance:
0/Biological Factors; 0/Catecholamines; 0/Culture Media; 0/Neurotoxins

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