Document Detail


No reduction of alpha-tocopherol quinone by glutathione in rat liver microsomes.
MedLine Citation:
PMID:  11266656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell membrane is protected against lipid peroxidation by endogenous antioxidants such as vitamin E (alpha-tocopherol). The oxidised form of alpha-tocopherol (alpha-tocopherol quinone) does not have this antioxidant function. However, the literature indicates that alpha-tocopherol quinone can be reduced to alpha-tocopherol in vivo and thereby will add to the total antioxidant potential (Moore AN, Ingold KU. Free Radic Biol Med 1997;22:931-4). We found that GSH (reduced glutathione) did not mediate the reduction of alpha-tocopherol quinone, either directly in solution or in rat liver microsomes fortified with alpha-tocopherol quinone. This renders GSH a less likely candidate for alpha-tocopherol quinone reduction in vivo. In addition, alpha-tocopherol quinone did not enhance GSH-dependent protection against lipid peroxidation, either in control microsomes, or in vitamin E-extracted microsomes. Indeed, alpha-tocopherol quinone blocked GSH-dependent protection against lipid peroxidation in vitamin E-extracted microsomes. This indicates that alpha-tocopherol quinone can act as a pro-oxidant.
Authors:
R I van Haaften; C T Evelo; G R Haenen; A Bast
Related Documents :
19427736 - Blockade of alpha 1a-adrenoceptor: a novel possible strategy for male contraception.
18691636 - Developmental enhancement of alpha2-adrenoceptor-mediated suppression of inhibitory syn...
2847126 - The role of alpha 2-adrenoceptors in the mechanism of action of antidepressant drugs.
12861436 - Nandrolone treatment decreases the level of rat kidney alpha(1b)-adrenoceptors.
19435506 - Regulation of catabolic gene expression in normal and degenerate human intervertebral d...
17575006 - Tnf-alpha induces muc1 gene transcription in lung epithelial cells: its signaling pathw...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  61     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-27     Completed Date:  2001-04-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  715-9     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Toxicology, Faculty of Medicine, Universiteit Maastricht, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. r.vanhaaften@farmaco.unimaas.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Glutathione / metabolism*
Lipid Peroxidation
Male
Microsomes, Liver / metabolism*
Oxidation-Reduction
Rats
Rats, Inbred Lew
Vitamin E / analogs & derivatives*,  metabolism*
Chemical
Reg. No./Substance:
1406-18-4/Vitamin E; 70-18-8/Glutathione; 7559-04-8/tocopherylquinone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of the glutathione/glutathione disulfide redox couple on thiopurine methyltransferase.
Next Document:  Debenzylation of O(6)-benzyl-8-oxoguanine in human liver: implications for O(6)-benzylguanine metabo...