| No loss of genomic imprinting of IGF-II and H19 in placentas of diabetic pregnancies with fetal macrosomia. | |
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MedLine Citation:
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PMID: 17306581 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Fetal macrosomia is a common complication of maternal diabetes mellitus and is associated with substantial morbidity, but the precise cellular and molecular mechanisms that induce fetal macrosomia are not well understood. The imprinted genes IGF-II and H19 are crucial for placental development and fetal growth. The term placentas from diabetic pregnancies express more insulin-like growth factor II (IGF-II) than those from normal pregnancies. Deregulation of their imprinting status is observed in the macrosomia-associated syndrome, the Beckwith-Wiedemann syndrome. The aim of this study was to determine whether loss of imprinting hence biallelic expression was also a hallmark of macrosomia in diabetic pregnancies. DESIGN AND METHODS: IGF-II and H19 maternal and paternal expressions were studied in placentas from two groups of type 1 diabetic mothers: one with macrosomic babies and the other with babies of normal weight. Maternal or paternal allele specific expressions were defined by using DNA polymorphic markers of the IGF-II and H19 genes. RFLP analysis was performed on PCR products from genomic DNA of the father, the mother and the child, and on RT-PCR products from placental mRNA. RESULTS: RFLP analysis showed that the IGF-II gene remains paternally expressed and the H19 gene remains maternally expressed in all placentas examined, independently of the birth weight status. CONCLUSIONS: These results suggest that, in contrast with Beckwith-Wiedemann syndrome-associated macrosomia, loss of imprinting for IGF-II or H19 is not a common feature of diabetic pregnancies associated with macrosomia. |
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Authors:
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A Vambergue; I Fajardy; P Dufour; A S Valat; M Vandersippe; P Fontaine; P M Danze; J Rousseaux |
Publication Detail:
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Type: Journal Article Date: 2007-02-15 |
Journal Detail:
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Title: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society Volume: 17 ISSN: 1096-6374 ISO Abbreviation: Growth Horm. IGF Res. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-10 Completed Date: 2007-07-05 Revised Date: 2011-10-07 |
Medline Journal Info:
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Nlm Unique ID: 9814320 Medline TA: Growth Horm IGF Res Country: Scotland |
Other Details:
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Languages: eng Pagination: 130-6 Citation Subset: IM |
Affiliation:
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Department of Endocrinology and Diabetes, Marc Linquette Hospital, CHRU Lille, France. a-vambergue@chru-lille.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis Diabetes Mellitus, Type 1 / genetics, metabolism* Female Fetal Macrosomia / genetics* Genomic Imprinting* Humans Infant, Newborn Insulin-Like Growth Factor II / genetics*, metabolism Placenta / chemistry, metabolism* Pregnancy Pregnancy in Diabetics / genetics, metabolism* RNA, Messenger / analysis, metabolism RNA, Untranslated / genetics*, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/H19 long non-coding RNA; 0/RNA, Messenger; 0/RNA, Untranslated; 67763-97-7/Insulin-Like Growth Factor II; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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