| No functional benefit for hDAF-transgenic rat livers despite protection from tissue damage following perfusion with human serum. | |
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MedLine Citation:
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PMID: 12478405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Currently, xenogeneic extracorporeal liver perfusion is used in the treatment of acute liver failure. In order to determine whether transgeneity for human regulatory proteins could improve the functional outcome of the ex-vivo liver in relation to the histopathological changes, we studied the effect of the humoral mechanism in xenogeneic isolated rat liver perfusion in normal and transgenic rat livers. Isolated rat liver perfusion was performed for 2 h in normal rat livers with Krebs Henseleit (KH) and human serum (HS), and in livers transgenic for human decay accelerating factor (hDAF; Tg HS). Function of the liver was established by measurement of liver enzymes and bile production, and clearance of bromosulphophthalein (BSP). Tissue specimens taken after perfusion were analysed by routine histology and immunofluorescence staining for C3c deposition. No change in release of liver enzymes could be established throughout the perfusion period. In the 2nd hour, a higher level of bile production was seen for the transgenic group than for the HS group. The transgenic rat livers outperformed the normal livers perfused with HS, when BSP concentration in the bile was measured; however, clearance of BSP from the perfusate was not significantly different. Haematoxylin-eosin (HE) staining of the liver tissue showed evidence of hyperacute rejection in the HS group. There was only mild tissue injury in the transgenic liver. High-intensity fluorescent staining for C3c deposition was seen in the normal livers perfused with HS, and significantly less in the transgenic livers. Although histologically less tissue damage and less C3c deposition was shown, no significantly improved function of the livers transgenic for hDAF was established. These results suggest that for short-term extracorporeal liver perfusion transgenesis offers no functional benefit. |
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Authors:
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Hein Stockmann; Caroline Verbakel; Petra Okkema; Fred Bonthuis; Severin Menoret; Ignacio Anegon; Richard Marquet; Jan IJzermans |
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Publication Detail:
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Type: Journal Article Date: 2002-10-19 |
Journal Detail:
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Title: Transplant international : official journal of the European Society for Organ Transplantation Volume: 15 ISSN: 0934-0874 ISO Abbreviation: Transpl. Int. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-12 Completed Date: 2003-09-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8908516 Medline TA: Transpl Int Country: Germany |
Other Details:
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Languages: eng Pagination: 595-601 Citation Subset: IM |
Affiliation:
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Department of General Surgery, University Hospital Dijkzigt, 3000 CA Rotterdam, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Antigens, CD / genetics Antigens, CD55 / genetics* Blood Physiological Phenomena Blood Transfusion Humans Liver / pathology, physiology* Male Perfusion / adverse effects Rats Rats, Sprague-Dawley Rats, Wistar Transplantation, Heterologous |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD55 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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