Document Detail


No evidence of HTLV-I proviral integration in lymphoproliferative disorders associated with cutaneous T-cell lymphoma.
MedLine Citation:
PMID:  9033279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several recent studies have reported detection of HTLV-I genetic sequences in patients with cutaneous T-cell lymphoma (CTCL) including mycosis fungoides and Sezary syndrome. The purpose of this study was to determine whether HTLV-I was detectable in lesional tissues of patients suffering from diseases known to be associated with CTCL. Thirty-five cases were obtained from diverse geographical locations including Ohio, California, Switzerland, and Japan. Six of them had concurrent CTCL. Cases were analyzed using a combination of genomic polymerase chain reaction (PCR)/ Southern blot, dot blot, and Southern blot analyses. All assays were specific for HTLV-I provirus. Sensitivity ranged from approximately 10(-6) for PCR-based studies to 10(-2) for unamplified genomic blotting. Lesional DNA from patients with lymphomatoid papulosis (fourteen cases), Hodgkin's disease (twelve cases), and CD30+ large-cell lymphoma (nine cases) was tested for the HTLV-I proviral pX region using a genomic PCR assay followed by confirmatory Southern blot analysis with a nested oligonucleotide pX probe. All cases were uniformly negative. All of the Hodgkin's disease cases, eight of the large-cell lymphoma cases, and six of the lymphomatoid papulosis cases were then subjected to dot blot analysis of genomic DNA using a full-length HTLV-I proviral DNA probe that spans all regions of the HTLV-I genome. Again, all cases were negative. Finally, eleven of the Hodgkin's disease cases were also subjected to Southern blot analysis of EcoRI-digested genomic DNA using the same full-length HTLV-I probe. Once again, all cases were negative. These findings indicated that, despite utilization of a variety of sensitive and specific molecular biological methods, HTLV-I genetic sequences were not detectable in patients with CTCL-associated lymphoproliferative disorders. These results strongly suggest that the HTLV-I retrovirus is not involved in the pathogenesis of these diseases.
Authors:
G S Wood; J M Schaffer; R Boni; R Dummer; G Burg; M Takeshita; M Kikuchi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  150     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-18     Completed Date:  1997-03-18     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  667-73     Citation Subset:  AIM; IM; X    
Affiliation:
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
DNA, Viral / analysis
Human T-lymphotropic virus 1 / genetics,  isolation & purification*
Humans
Lymphoma, T-Cell, Cutaneous / complications*
Lymphoproliferative Disorders / complications*,  genetics,  virology*
Polymerase Chain Reaction
Proviruses / genetics,  isolation & purification*
Grant Support
ID/Acronym/Agency:
AR39750/AR/NIAMS NIH HHS; AR40844/AR/NIAMS NIH HHS; CA60014/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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