Document Detail

No effect of cigarette smoking dose on oxidized plasma proteins.
MedLine Citation:
PMID:  17996865     Owner:  NLM     Status:  MEDLINE    
Cigarette smoking is a major source of oxidative stress. Protein carbonyls have been used as a biomarker of oxidative stress because of the relative stability of carbonylated proteins and the high protein concentration in blood. Increased levels of carbonyl groups have been found in serum proteins of smokers compared to nonsmokers. However, neither the dose effect of current cigarette smoke nor other predictors of oxidative stress have been studied. Hence, we used an Enzyme-Linked Immunosorbent Assay (ELISA) to evaluate plasma protein carbonyls in smokers recruited in the Early Lung Cancer Action Project (ELCAP) program. The lung cancer screening program enrolled current and former smokers age 60 years and over without a prior cancer diagnosis. A total of 542 participants (282 men and 260 women) completed a baseline questionnaire and provided blood samples for the biomarker study. Protein oxidation was measured by derivatization of the carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) and ELISA quantitation of the DNPH group. Current smoking status was confirmed with urinary cotinine. The mean (+/-S.D.) protein carbonyl level was 17.9+/-2.9 nmol carbonyl/ml plasma. Protein carbonyls did not differ significantly by gender. Carbonyl levels were higher among current than former smokers, but these differences did not attain statistical significance, nor did differences by urine cotinine levels, pack-years, pack/day among current smokers, and smoking duration. In a multiple regression analysis, higher protein carbonyl levels were independently associated with increasing age (0.59 nmol/ml increase per 10 years, 95% CI 0.14, 1.05, p=0.01), African-American vs. white race/ethnicity, (1.30 nmol/ml, 95% CI 0.4, 2.19, p=0.008), and lower educational attainment (0.75 nmol/ml, 95% CI 0.12, 1.38, p=0.02). Although we found no significant difference between current vs. past cigarette smoking and protein carbonyls in this older group of smokers, associations were found for age, ethnicity, and educational attainment. Our results indicate that the measurement of plasma carbonyls by this ELISA technique is still an easy and suitable method for studies of diseases related to oxidative stress.
Chih-Ching Yeh; R Graham Barr; Charles A Powell; Sonia Mesia-Vela; Yuanjia Wang; Nada K Hamade; John H M Austin; Regina M Santella
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2007-11-09
Journal Detail:
Title:  Environmental research     Volume:  106     ISSN:  0013-9351     ISO Abbreviation:  Environ. Res.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-03-28     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-25     Citation Subset:  IM    
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MeSH Terms
Biological Markers / blood
Enzyme-Linked Immunosorbent Assay
Hydrazines / blood
Lung Neoplasms / blood,  epidemiology,  etiology,  prevention & control*
Mass Screening
Middle Aged
New York City / epidemiology
Oxidative Stress*
Protein Carbonylation / physiology*
Smoking / blood*
Grant Support
CA013696/CA/NCI NIH HHS; ES09089/ES/NIEHS NIH HHS; HL075476/HL/NHLBI NIH HHS; P30 CA013696/CA/NCI NIH HHS; P30 CA013696-319033/CA/NCI NIH HHS; P30 ES009089/ES/NIEHS NIH HHS; P30 ES009089-096522/ES/NIEHS NIH HHS; R01 CA120174/CA/NCI NIH HHS; R01 CA120174-02/CA/NCI NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Hydrazines; 0/dinitrophenylhydrazine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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