Document Detail


No detection of enteroviral genome in the myocardium of patients with arrhythmogenic right ventricular cardiomyopathy.
MedLine Citation:
PMID:  10889821     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Despite the evidence of familial occurrence, chromosomal gene mapping, and apoptosis as a mechanism of myocyte death, the aetiopathogenesis of arrhythmogenic right ventricular cardiomyopathy (ARVC) remains speculative. Because of the frequent histological finding of focal inflammatory infiltrates, the hypothesis of an infective myocarditis aetiology has been put forward. The aim of this investigation was to test this hypothesis. The presence of enteroviruses was investigated by a highly sensitive and specific molecular technique. METHODS: Endomyocardial tissue samples from 20 patients with ARVC (11 male, nine female; mean age, 40 years; SD, 16) and 20 control subjects with other cardiac diseases were analysed using reverse transcription and nested polymerase chain reaction (PCR). Myocardial samples obtained from four patients with enteroviral myocarditis and coxsackie B3 virus infected cells were used as positive controls. RESULTS: Endomyocardial biopsy was diagnostic for ARVC in all patients: myocardial atrophy was seen, with less than 45% residual myocytes. Foci of inflammatory infiltrates were seen in four biopsies, and the cells were identified by immunohistochemistry as mainly T cells. All samples, from both patients with ARVC and subjects with other cardiac diseases, were negative for enteroviral genome by means of nested PCR. CONCLUSION: These findings indicate that enteroviruses are not involved in the aetio-pathogenesis of ARVC. Future molecular studies should investigate the presence of other infective agents, as well as their possible role in triggering apoptosis.
Authors:
F Calabrese; A Angelini; G Thiene; C Basso; A Nava; M Valente
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  53     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-14     Completed Date:  2000-07-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  382-7     Citation Subset:  AIM; IM    
Affiliation:
Institute of Pathological Anatomy, University of Padua, Padova, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Arrhythmogenic Right Ventricular Dysplasia / pathology,  virology*
Biopsy
Endocardium / pathology,  virology*
Enterovirus / genetics,  isolation & purification*
Female
Genome, Viral
Humans
Male
Middle Aged
RNA, Viral / analysis
Reverse Transcriptase Polymerase Chain Reaction / methods
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/RNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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