Document Detail


No detectable cytomegalovirus and Epstein-Barr virus genomes in the pancreas of recent-onset IDDM patients.
MedLine Citation:
PMID:  7672487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Viral infection is assumed to trigger or exacerbate autoimmune responses against pancreatic beta cells leading to the development of insulin-dependent diabetes mellitus (IDDM). We therefore examined by polymerase chain reaction the presence of two candidate viruses, cytomegalovirus and Epstein-Barr virus, in IDDM pancreases. Pancreas tissues were obtained by biopsy under laparoscopy from 16 recent-onset IDDM patients: age 17-53 years; disease duration 0-7 months; six had flu-like symptoms before onset. Frozen sections were made and subjected to DNA amplification. DNA samples were prepared from the frozen sections and polymerase chain reaction was performed using primers specific to cytomegalovirus, Epstein-Barr virus and control gene for HLA-DP. Cytomegalovirus- and Epstein-Barr virus-infected cells were used for positive control. Southern blot analysis could detect cytomegalovirus DNA from as few as 2 x 10(-1) cytomegalovirus-infected cells and Epstein-Barr virus DNA from two Epstein-Barr virus-infected cells. This highly sensitive analysis, however, could not detect cytomegalovirus or Epstein-Barr virus genomes in pancreases of recent-onset IDDM. A single copy human gene (HLA-DP) was amplified from all IDDM pancreases indicating that DNA amplification was performed without inhibition. We conclude that cytomegalovirus or Epstein-Barr virus genomes are unlikely to exist in pancreas biopsy specimens of recent-onset IDDM patients.
Authors:
N Itoh; T Hanafusa; K Yamagata; H Nakajima; K Tomita; S Tamura; M Inada; S Kawata; N Kono; M Kuwajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetologia     Volume:  38     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-13     Completed Date:  1995-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  667-71     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Osaka University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Base Sequence
Biopsy
Cytomegalovirus / genetics,  isolation & purification*
DNA Primers
Diabetes Mellitus, Type 1 / pathology,  virology*
Genes, MHC Class II
Genome, Viral*
HLA-DP Antigens / genetics
Herpesvirus 4, Human / genetics,  isolation & purification*
Humans
Middle Aged
Molecular Sequence Data
Pancreas / pathology,  virology*
Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/HLA-DP Antigens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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