Document Detail


Nkx3.1 and p27(KIP1) cooperate in proliferation inhibition and apoptosis induction in human androgen-independent prostate cancer cells.
MedLine Citation:
PMID:  19266349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prostate cancer (PC), which responds well to androgen ablation initially, invariably progresses to treatment resistance. The so-called androgen-independent PC is also a concern, since there is no effective therapy so far. Nkx3.1 is a putative prostate tumor suppressor that is expressed exclusively in the prostate under the regulation of androgen, and p27(KIP1) functions as a cell proliferation inhibitor and apoptosis trigger by disrupting the cyclin-dependent kinase (CDK)-cyclin complex. Lack of expressions of Nkx3.1 and/or p27(KIP1) have been detected in most advanced PC and is associated with poor clinical progression. Here, we show that endogenous expressions of both Nkx3.1 and p27(KIP1) are lost in the androgen-independent PC3 PC cells, while remaining intact in LNCaP PC cells, which contain functional androgen receptor (AR) and are hormone-responsive. Ectopic restoration of either Nkx3.1 or p27(KIP1) in PC3 cells results in reduced cell proliferation, and increased cell death. Both effects are synergistically enhanced when the two molecules are coexpressed. p27(KIP1) overexpression in PC3 results in increased cell population ceased at the G0/G1 phase, and this cell-cycle-arresting effect is significantly enhanced by the coexpression of Nkx3.1. Flow cytometry further revealed that Nkx3.1 and p27(KIP1) also cooperatively render more PC3 cells undergoing apoptosis. Consistently, the coexpression of Nkx3.1 and p27(KIP1) leads to the decreased expression of Bcl-2 oncogene and a concomitantly upregulated Bax expression. It also activates caspase 3 and leads to increased cleavage of PARP. Our findings thus reveal the crucial relevance of the combined antiproliferative and proapoptotic activities of Nkx3.1 and p27(KIP1) in androgen-independent PC cells, and further suggest that a combined, rather than single gene manipulation may be of clinical value for hormone-refractory PC.
Authors:
Ping Wang; Qi Ma; JinDan Luo; Ben Liu; FuQing Tan; ZhiGen Zhang; ZhaoDian Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer investigation     Volume:  27     ISSN:  1532-4192     ISO Abbreviation:  Cancer Invest.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-09     Completed Date:  2009-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307154     Medline TA:  Cancer Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-75     Citation Subset:  IM    
Affiliation:
Department of Urology, The First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Androgens / metabolism
Apoptosis*
Cell Cycle
Cell Line, Tumor
Cell Proliferation*
Gene Expression Regulation, Neoplastic
Homeodomain Proteins / genetics,  metabolism*
Humans
Intracellular Signaling Peptides and Proteins / genetics,  metabolism*
Male
Prostatic Neoplasms / genetics,  metabolism*,  pathology
Proto-Oncogene Proteins c-bcl-2 / metabolism
Signal Transduction
Time Factors
Transcription Factors / genetics,  metabolism*
Transfection
Young Adult
bcl-2-Associated X Protein / metabolism
Chemical
Reg. No./Substance:
0/Androgens; 0/BAX protein, human; 0/CDKN1B protein, human; 0/Homeodomain Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/NKX3-1 protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/Transcription Factors; 0/bcl-2-Associated X Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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