| Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine. | |
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MedLine Citation:
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PMID: 18022152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nkx2.2 is a homeodomain-containing transcription factor essential for pancreatic islet cell specification. In this study we investigate the role of Nkx2.2 within the small intestine. We have determined that Nkx2.2 is expressed at the onset of intestinal epithelial cell differentiation in specific intestinal cell populations, including a subset of enteroendocrine cells. Similar to its role in the pancreatic islet, Nkx2.2 regulates cell fate choices within the intestinal enteroendocrine population; in the Nkx2.2 null mice, several hormone-producing enteroendocrine cell populations are absent or reduced and the ghrelin-producing cell population is upregulated. The remaining intestinal cell populations, including the paneth cells, goblet cells, and enterocytes appear to be unaffected by the loss of Nkx2.2. Furthermore, similar to the pancreatic islet, Nkx2.2 appears to function upstream of Pax6 in regulating intestinal cell fates; Pax6 mRNA and protein expression is decreased in the Nkx2.2 null mice. These studies identify a novel role for Nkx2.2 in intestinal endocrine cell development and reveal the regulatory similarities between cell type specification in the pancreatic islet and in the enteroendocrine population of the intestine. |
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Authors:
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Shailey Desai; Zoe Loomis; Aimee Pugh-Bernard; Jessica Schrunk; Michelle J Doyle; Angela Minic; Erica McCoy; Lori Sussel |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-10-03 |
Journal Detail:
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Title: Developmental biology Volume: 313 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-27 Completed Date: 2008-01-25 Revised Date: 2012-06-04 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 58-66 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Genetics, University of Colorado at Denver Health Sciences Center, Aurora, CO 80045, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Lineage* Endocrine Glands / cytology*, physiology Gene Expression Regulation, Developmental Homeodomain Proteins / physiology* Intestine, Small / cytology*, physiology* Mice Transcription Factors / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P30 DK57516/DK/NIDDK NIH HHS; R01 DK082590/DK/NIDDK NIH HHS; R21 DK061151-01/DK/NIDDK NIH HHS; U01 DK072504-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/Nkx-2.2 homedomain protein; 0/Transcription Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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