Document Detail


Nkx2.2 regulates cell fate choice in the enteroendocrine cell lineages of the intestine.
MedLine Citation:
PMID:  18022152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nkx2.2 is a homeodomain-containing transcription factor essential for pancreatic islet cell specification. In this study we investigate the role of Nkx2.2 within the small intestine. We have determined that Nkx2.2 is expressed at the onset of intestinal epithelial cell differentiation in specific intestinal cell populations, including a subset of enteroendocrine cells. Similar to its role in the pancreatic islet, Nkx2.2 regulates cell fate choices within the intestinal enteroendocrine population; in the Nkx2.2 null mice, several hormone-producing enteroendocrine cell populations are absent or reduced and the ghrelin-producing cell population is upregulated. The remaining intestinal cell populations, including the paneth cells, goblet cells, and enterocytes appear to be unaffected by the loss of Nkx2.2. Furthermore, similar to the pancreatic islet, Nkx2.2 appears to function upstream of Pax6 in regulating intestinal cell fates; Pax6 mRNA and protein expression is decreased in the Nkx2.2 null mice. These studies identify a novel role for Nkx2.2 in intestinal endocrine cell development and reveal the regulatory similarities between cell type specification in the pancreatic islet and in the enteroendocrine population of the intestine.
Authors:
Shailey Desai; Zoe Loomis; Aimee Pugh-Bernard; Jessica Schrunk; Michelle J Doyle; Angela Minic; Erica McCoy; Lori Sussel
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-10-03
Journal Detail:
Title:  Developmental biology     Volume:  313     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-27     Completed Date:  2008-01-25     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  58-66     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver Health Sciences Center, Aurora, CO 80045, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Lineage*
Endocrine Glands / cytology*,  physiology
Gene Expression Regulation, Developmental
Homeodomain Proteins / physiology*
Intestine, Small / cytology*,  physiology*
Mice
Transcription Factors / physiology*
Grant Support
ID/Acronym/Agency:
P30 DK57516/DK/NIDDK NIH HHS; R01 DK082590/DK/NIDDK NIH HHS; R21 DK061151-01/DK/NIDDK NIH HHS; U01 DK072504-01/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Nkx-2.2 homedomain protein; 0/Transcription Factors
Comments/Corrections

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