Document Detail


Nitrosative stress causes amino acid auxotrophy in hmp mutant Salmonella Typhimurium.
MedLine Citation:
PMID:  21752086     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cytotoxic nitic oxide (NO) damages various bacterial macromolecules, resulting in abnormal metabolism by mechanisms largely unknown. We show that NO can cause amino acid auxotrophy in Salmonella Typhimurium lacking major NO-metabolizing enzyme, Flavohemoglobin Hmp. In NO-producing cultures, supplementation with amino acid pool restores growth of Hmp-deficient Salmonella to normal growth phases, whereas excluding Cys or branched-chain amino acids (BCAA) Leu, Ile, or Val from amino acid pool reduces growth recovery. Data suggest that, without detoxification, NO might inactivate key enzymes in the biosynthesis pathway of amino acids essential for Salmonella replication in amino acid-limiting host environments.
Authors:
Yoon Mee Park; Hee Jeong Park; Young Hee Joung; Iel Soo Bang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-13
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Microbiology and Immunology, Chosun University School of Dentistry, Gwangju 501-759 School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
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