Document Detail

Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.
MedLine Citation:
PMID:  19378102     Owner:  NLM     Status:  MEDLINE    
Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.
Harshan Pisharath; Michael J Parsons
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  546     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-20     Completed Date:  2009-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-43     Citation Subset:  IM    
Department of Surgery, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
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MeSH Terms
Animals, Genetically Modified / embryology,  genetics,  metabolism*
Anti-Infective Agents / pharmacology
Apoptosis / drug effects*
DNA / genetics,  metabolism
Escherichia coli Proteins / administration & dosage*,  biosynthesis
Genetic Engineering / methods
Green Fluorescent Proteins
Metronidazole / pharmacology
Microscopy, Confocal
Nitroreductases / administration & dosage*,  biosynthesis
Prodrugs / pharmacology
Recombinant Fusion Proteins / administration & dosage,  biosynthesis
Zebrafish / embryology,  genetics*,  metabolism*
Grant Support
NCRR T32 07002//PHS HHS
Reg. No./Substance:
0/Anti-Infective Agents; 0/Escherichia coli Proteins; 0/Prodrugs; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 443-48-1/Metronidazole; 9007-49-2/DNA; EC 1.7.-/NfnB protein, E coli; EC 1.7.-/Nitroreductases

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