Document Detail

Nitroprusside selectively reduces ventricular preload in the stage 21 chick embryo.
MedLine Citation:
PMID:  8681337     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Embryonic cardiovascular function is dynamically regulated at the tissue level. Nitric oxide (NO) regulates vascular tone and influences cardiovascular function in neonatal and mature circulations. However, the role of NO in regulating embryonic cardiovascular function is undefined. We hypothesized that NO released from nitroprusside alters embryonic vascular tone with secondary effects on ventricular function. METHODS: We measured ventricular pressure and calculated ventricular volume from area using ellipsoid geometry for 180 s after suffusion of 0.0, 0.1, 1.0, or 2.5 micrograms of nitroprusside in 10 microliters of KHB buffer, or after acute venous hemorrhage in stage 21 chick embryos (n > or = 8 per group). We plotted pressure-volume loops and analyzed standard parameters of cardiovascular function by ANOVA, regression analysis, and ANCOVA. RESULTS: Following nitroprusside, heart rate was unchanged, end-diastolic volume (EDV), stroke volume (SV), and end-systolic pressure decreased (P < 0.05 at 180 s). For 1.0 microgram of nitroprusside, EDV decreased by 27 +/- 6%, SV decreased by 36 +/- 6%, and end-systolic pressure decreased by 28 +/- 3%. The EDV vs. SV relationship was unchanged with the exception of the 2.5 micrograms nitroprusside dose. Arterial elastance was unchanged with the exception of the 2.5 micrograms nitroprusside dose. The EDV vs. SV relationship and arterial elastance during preload reduction suggest that ventricular afterload did not decrease following NO. CONCLUSIONS: In contrast to the mature circulation, NO reduced preload without decreasing ventricular afterload. Thus, vasoactive agents may have unique roles in the regulation of cardiovascular structure and function during embryogenesis.
P N Bowers; J P Tinney; B B Keller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cardiovascular research     Volume:  31 Spec No     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-08-20     Completed Date:  1996-08-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  E132-8     Citation Subset:  IM    
NIH SCOR in Pediatric Cardiovascular Diseases, Strong Children's Research Center, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
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MeSH Terms
Blood Pressure / drug effects
Chick Embryo
Heart / drug effects,  embryology*
Morphogenesis / physiology
Nitric Oxide / physiology
Nitroprusside / pharmacology*
Statistics, Nonparametric
Stroke Volume / drug effects
Vasodilator Agents / pharmacology*
Grant Support
Reg. No./Substance:
0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside

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