Document Detail

Nitrogenase activity in wheat seedlings bearing para-nodules induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and inoculated with Azospirillum.
MedLine Citation:
PMID:  1299838     Owner:  NLM     Status:  MEDLINE    
Nitrogenase activity (C2H2 reduction) was demonstrated in seedlings of wheat roots bearing para-nodules induced by 2,4-dichlorophenoxyacetic acid (2,4-D) and inoculated with Azospirillum brasilense. Increased nitrogenase activity was observed in inoculated para-nodulated seedlings as compared to inoculated roots not treated by 2,4-D under the conditions of assay used. 2,4-D had no stimulating effect on plant ethylene production in the absence of acetylene. When inoculation was performed with a Nif-mutant of A. brasilense, no ethylene production was detected. It was also shown that the energy source required for nitrogenase activity was supplied by the host plant.
A M Zeman; Y T Tchan; C Elmerich; I R Kennedy
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research in microbiology     Volume:  143     ISSN:  0923-2508     ISO Abbreviation:  Res. Microbiol.     Publication Date:    1992 Nov-Dec
Date Detail:
Created Date:  1993-05-19     Completed Date:  1993-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907468     Medline TA:  Res Microbiol     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  847-55     Citation Subset:  IM    
Department of Agricultural Chemistry, University of Sydney, Australia.
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MeSH Terms
2,4-Dichlorophenoxyacetic Acid / pharmacology*
Acetylene / metabolism
Azospirillum brasilense / drug effects,  enzymology,  metabolism*
Ethylenes / metabolism
Microscopy, Electron
Nitrogen Fixation / physiology
Nitrogenase / metabolism*
Symbiosis / physiology*
Triticum / drug effects,  enzymology,  microbiology*
Reg. No./Substance:
0/Ethylenes; 74-86-2/Acetylene; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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