Document Detail


Nitrogen metabolism in the perfused cirrhotic liver of the rat.
MedLine Citation:
PMID:  1113014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isolated perfused liver of the cirrhotic rat produces greater quantities of ammonia from endogenous sources or from added alanine than do control livers. It also fails to take up ammonia. In these cirrhotic livers ethanol increases ammonia production in the presence of alanine. When cirrhotic rats had received a normal diet for 10 days before perfusion, the output of ammonia from endogenous sources and from added alanine is partially restored to normal. The addition of ethanol in the presence of alanine to the perfusate of these cirrhotic livers then results in an increased rate of production of glutamine rather than of ammonia. A normal diet given these cirrhotic animals restores the uptake of ammonia to that seen in the control animals but, unlike the normal animals, the addition of ornithine increases neither the uptake of ammonia nor the output of urea. In the normal animals this effect of ornithine on the uptake of ammonia is abolished by ethanol. It is concluded that cirrhotic rat liver is a source of endogenous ammonia and that the magnitude of that contribution is modified by diet and by ethanol.
Authors:
K S Henley; P E Clancy; E G Laughrey; L G Lyra
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of laboratory and clinical medicine     Volume:  85     ISSN:  0022-2143     ISO Abbreviation:  J. Lab. Clin. Med.     Publication Date:  1975 Feb 
Date Detail:
Created Date:  1975-05-19     Completed Date:  1975-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375375     Medline TA:  J Lab Clin Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / metabolism
Ammonia / metabolism
Animals
Diet
Ethanol / pharmacology
Glucose / metabolism
Glutamates / metabolism
Glutamine / biosynthesis
Liver / anatomy & histology,  metabolism
Liver Cirrhosis, Experimental / metabolism*
Male
Nitrogen / metabolism*
Organ Size
Ornithine / pharmacology
Perfusion
Rats
Urea / metabolism
Chemical
Reg. No./Substance:
0/Glutamates; 50-99-7/Glucose; 56-41-7/Alanine; 56-85-9/Glutamine; 57-13-6/Urea; 64-17-5/Ethanol; 7006-33-9/Ornithine; 7664-41-7/Ammonia; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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